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6737 Mowry Ave
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H2AFX / H2AX

H2A histone family, member X

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Gene Name: H2A histone family, member X
Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone
Target Sequences: NM_002105 NP_002096.1 P16104

Publications (13)

1
Splicing regulator SC35 is essential for genomic stability and cell proliferation during mammalian organogenesis. Xiao R, Sun Y, Ding JH, Lin S, Rose DW, Rosenfeld MG, Fu XD, Li X. Molecular and cellular biology. 2007 27:5393-402. [PubMed:17526736] [PMC:PMC1952092] Related Antibodies: LS-C41114.
2
Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age. Rossi DJ, Bryder D, Seita J, Nussenzweig A, Hoeijmakers J, Weissman IL. Nature. 2007 447:725-9. [PubMed:17554309] Related Antibodies: LS-C41114.
3
DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena. Mochizuki K, Novatchkova M, Loidl J. Journal of cell science. 2008 121:2148-58. [PubMed:18522989] [PMC:PMC3184542] Related Antibodies: LS-C41114.
4
Differential roles of telomere attrition in type I and II endometrial carcinogenesis. Akbay EA, Contreras CM, Perera SA, Sullivan JP, Broaddus RR, Schorge JO, Ashfaq R, Saboorian H, Wong KK, Castrillon DH. The American journal of pathology. 2008 173:536-44. [PubMed:18599611] [PMC:PMC2475790] Related Antibodies: LS-C41114.
5
Tetrahymena meiotic nuclear reorganization is induced by a checkpoint kinase-dependent response to DNA damage. Loidl J, Mochizuki K. Molecular biology of the cell. 2009 20:2428-37. [PubMed:19297526] [PMC:PMC2675622] Related Antibodies: LS-C41114.
6
gamma-H2AX foci as a biomarker for patient X-ray exposure in pediatric cardiac catheterization: are we underestimating radiation risks?. Beels L, Bacher K, De Wolf D, Werbrouck J, Thierens H. Circulation. 2009 120:1903-9. [PubMed:19858412]
7
Tumor regression and curability of preclinical neuroblastoma models by PEGylated SN38 (EZN-2208), a novel topoisomerase I inhibitor. Pastorino F, Loi M, Sapra P, Becherini P, Cilli M, Emionite L, Ribatti D, Greenberger LM, Horak ID, Ponzoni M. Clinical cancer research : an official journal of the American Association for Cancer Research. 2010 16:4809-21. (IHC-Fr; Mouse) [PubMed:20702613]
8
Reduced NR4A gene dosage leads to mixed myelodysplastic/myeloproliferative neoplasms in mice. Ramirez-Herrick AM, Mullican SE, Sheehan AM, Conneely OM. Blood. 2011 117:2681-90. [PubMed:21205929] [PMC:PMC3062356]
9
The SET2-RPB1 interaction domain of human RECQ5 is important for transcription-associated genome stability. Li M, Xu X, Liu Y. Molecular and cellular biology. 2011 31:2090-9. [PubMed:21402780] [PMC:PMC3133350]
10
The nucleolus directly regulates p53 export and degradation. Boyd MT, Vlatkovic N, Rubbi CP. The Journal of cell biology. 2011 194:689-703. [PubMed:21893597] [PMC:PMC3171122]
11
Variant repeats are interspersed throughout the telomeres and recruit nuclear receptors in ALT cells. Conomos D, Stutz MD, Hills M, Neumann AA, Bryan TM, Reddel RR, Pickett HA. The Journal of cell biology. 2012 199:893-906. (ICC; Human) [PubMed:23229897] [PMC:PMC3518223]
12
Ionizing Radiation-Induced -H2AX Activity in Whole Blood Culture and the Risk of Lung Cancer. He Y, Gong Y, Lin J, Chang DW, Gu J, Roth JA, Wu X. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2013 22:443-51. [PubMed:23300022] [PMC:PMC3601549]
13
MRPL35 Is Up-Regulated in Colorectal Cancer and Regulates Colorectal Cancer Cell Growth and Apoptosis. Litao Zhang, Peifen Lu, Lihong Yan, Lijun Yang, Yutao Wang, Junjun Chen, Jie Dai, Yahui Li, Zhiming Kang, Tao Bai, Yanfeng Xi, Jun Xu, Gongqin Sun, Tao Yang. The American journal of pathology. 2019 May;;189:1105-1120. [Full Text Article] [PubMed:30862482] Related Antibodies: LS-C348689.
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H2AFX / H2AX Antibody - Untreated Hela cells (Panel A), or overnight nocodazole treated Hela cells (Panel B) stained with purified mouse monoclonal antibody against Ser139 phosphorylated H2A.X, followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI.
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H2AFX / H2AX Antibody (phospho-Ser139, clone 2F3) LS-C41114
(4.4) Rating
(5) Publication(s)
H2AFX / H2AX Mouse anti-Human Monoclonal (pSer139) (2F3) Antibody
Human
ICC, IF, WB
Unconjugated
25 µg/$287; 100 µg/$401
H2AFX / H2AX Antibody - Detection of Human H2AX by Immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
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H2AFX / H2AX Antibody (C-Terminus) LS-C290487
H2AFX / H2AX Goat anti-Human Polyclonal (C-Terminus) Antibody
Mouse, Human
IHC, WB
Unconjugated
10 µl/$295; 100 µl/$514
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H2AFX / H2AX Antibody (phospho-Ser139, clone 20E3) LS-C348689
(4.0) Rating
(1) Publication(s)
H2AFX / H2AX Rabbit anti-Human Monoclonal (pSer139) (20E3) Antibody
Mouse, Rat, Monkey
Flo, IF, IHC, WB
Unconjugated
100 µl/$689
H2AFX / H2AX Antibody - IHC of p-Histone H2A.X (S139) pAb in paraffin-embedded human brain tissue.
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H2AFX / H2AX Antibody (phospho-Ser139) LS-C178034
H2AFX / H2AX Rabbit anti-Human Polyclonal (pSer139) Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$328; 100 µl/$419
H2AFX / H2AX ELISA Kit
Select
Mouse/Human/Rat Phospho-H2AFX / H2AX (Ser139) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1529
Cell-Based Phosphorylation
96-Well Microplate
Mouse, Human, Rat
Colorimetric - 450nm (TMB)
Adherent Cell Cultures
1 Plate/$668
H2AFX / H2AX Antibody - Detection of Human gamma-H2AX by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-gamma-H2AX ( Lot12) used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
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H2AFX / H2AX Antibody (phospho-Ser140) LS-C287018
H2AFX / H2AX Rabbit anti-Human Polyclonal (pSer140) Antibody
Mouse, Human
ICC, IF, IHC, WB
Unconjugated
10 µl/$295; 100 µl/$533
H2AFX / H2AX Antibody - Anti-H2AX antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ H2AFX / H2AX Antibody (C-Terminus) LS-B3282
(4.0) Rating
H2AFX / H2AX Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
IF, IHC, IHC-P, WB
Unconjugated
50 µg/$485
H2AFX / H2AX ELISA Kit
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Human H2AFX / H2AX (Sandwich ELISA) ELISA Kit - LS-F17627
Sandwich
96-Well Strip Plate
Human
78 - 5000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$669
H2AFX / H2AX Antibody - Immunofluorescence of monoclonal antibody to H2AFX on HeLa cell . [antibody concentration 10 ug/ml]
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H2AFX / H2AX Antibody (clone 3F4) LS-C197392
H2AFX / H2AX Mouse anti-Human Monoclonal (3F4) Antibody
Human
ELISA, IF
Unconjugated
100 µg/$423
H2AFX / H2AX Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PathPlus™ H2AFX / H2AX Antibody LS-B16720
Cancer
(5.0) Rating
H2AFX / H2AX Rabbit anti-Mouse Polyclonal Antibody
Mouse, Rat
IHC-P, WB
Unconjugated
50 µg/$460
H2AFX / H2AX ELISA Kit
Select
Mouse H2AFX / H2AX (Sandwich ELISA) ELISA Kit - LS-F17626
Sandwich
96-Well Strip Plate
Mouse
31.2 - 2000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates
1 Plate/$669
H2AFX / H2AX Antibody - Immunofluorescence staining of HepG2 cells at a dilution of 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
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H2AFX / H2AX Antibody (phospho-Tyr142) LS-C670391
H2AFX / H2AX Rabbit anti-Human Polyclonal (pTyr142) Antibody
Human
ELISA, IF
Unconjugated
50 µl/$298; 100 µl/$370
H2AFX / H2AX Antibody - Western Blot Positive WB detected in:293 whole cell lysate All Lanes:Phospho-Histone H2AX (S139) antibody at 0.23µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 KDa Observed band size: 15 KDa
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H2AFX / H2AX Antibody LS-C675312
H2AFX / H2AX Rabbit anti-Human Monoclonal Antibody
Human
ELISA, WB
Unconjugated
50 µl/$316; 100 µl/$386
H2AFX / H2AX Antibody - Immunohistochemistry image at a dilution of 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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H2AFX / H2AX Antibody LS-C670395
H2AFX / H2AX Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$298; 100 µl/$370
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H2AFX / H2AX Antibody (FITC) LS-C211528
H2AFX / H2AX Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
H2AFX / H2AX Antibody - Detection of Human H2AX by Immunohistochemistry. Sample: FFPE section of human colon adenocarcinoma. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:500. Detection: DAB.
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H2AFX / H2AX Antibody (C-Terminus) LS-C285928
H2AFX / H2AX Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Mouse, Human
IHC
Unconjugated
1 µg/$295; 100 µl/$532
H2AFX / H2AX Antibody - Immunohistochemical analysis of Histone H2A.X staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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H2AFX / H2AX Antibody (C-Terminus) LS-C352308
H2AFX / H2AX Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Rabbit, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$299
H2AFX / H2AX Antibody - Immunohistochemical analysis of Histone H2A.X staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
H2AFX / H2AX Antibody (C-Terminus) LS-C358767
H2AFX / H2AX Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Mouse, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
30 µl/$255; 100 µl/$299; 200 µl/$351
Select
H2AFX / H2AX Antibody (phospho-Ser139, clone 20E3, PE) LS-C183907
H2AFX / H2AX Rabbit anti-Human Monoclonal (pSer139) (PE) (20E3) Antibody
Mouse, Rat, Human, Monkey
Flo
PE Conjugated
100 µl/$680
Select
H2AFX / H2AX Antibody (C-Terminus) LS-C153461
H2AFX / H2AX Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$440; 100 µg/$480
Select
H2AFX / H2AX Antibody (phospho-Ser139) LS-C7424
H2AFX / H2AX Mouse anti-Human Monoclonal (pSer139) Antibody
Rabbit, Dog, Bovine, Human, Monkey
ICC, IF, IP, WB
Unconjugated
100 µg/$725
H2AFX / H2AX Antibody - Western blot of Histone H2A.X antibody
Select
H2AFX / H2AX Antibody (80-160 aa) LS-C383940
H2AFX / H2AX Rabbit anti-Human Polyclonal (80-160 aa) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
H2AFX / H2AX Antibody - Anti-H2AX antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
H2AFX / H2AX Antibody (C-Terminus) LS-B6918
H2AFX / H2AX Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded rat liver using H2AFX antibody at dilution of 1:100 (40x lens).
Select
H2AFX / H2AX Antibody LS-C746881
H2AFX / H2AX Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
H2AFX / H2AX Antibody - Immunohistochemistry of paraffin-embedded human stomach using H2AFX antibody at dilution of 1:100 (40x lens).
Select
H2AFX / H2AX Antibody LS-C746951
H2AFX / H2AX Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
Viewing 1-25 of 114 product results



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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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