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Catalog Number	Size	Price
LS-C670395-50	50 µl	$298
LS-C670395-100	100 µl	$370

H2AFX / H2AX Antibody - Immunohistochemistry image at a dilution of 1:50 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

H2AFX / H2AX Antibody - Immunohistochemistry image at a dilution of 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

H2AFX / H2AX Antibody - Immunofluorescence staining of hela with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .

H2AFX / H2AX Antibody - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate. All lanes: H2AFX antibody at 1.64 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 16 KDa. Observed band size: 16 KDa

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Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (IHC, IF, WB) LS‑C670395

Name: Anti-H2AFX / H2AX Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C670395-50
Price: 298.0000 USD
Availability: InStock

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Antibody:

H2AFX / H2AX Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, IF, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$298

LS-C670395-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

H2AFX / H2AX Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, IF, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

H2AX antibody LS-C670395 is an unconjugated rabbit polyclonal antibody to human H2AX (H2AFX). Validated for ELISA, IF, IHC and WB.

Target

Human H2AFX / H2AX

Synonyms

See All H2AFX / H2AX Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Protein G purified

Modifications

Unmodified

Immunogen

Peptide sequence around site of Ser (139) derived from human Histone H2AX.

Applications

IHC
IHC - Paraffin
Immunofluorescence
Western blot
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About H2AFX / H2AX

Uniprot: P16104 NCBI: NM_002105 NP_002096.1