Human H2AFX / H2AX

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide corresponding to a region within the C-Terminus of human H2AX.

C-Terminus

Recognizes human histone H2AX. H2AX comprises approximately 2-25% of the histone H2A pool in mammalian cells. The extended C-terminal tail of H2AX contains a serine (Ser139) embedded in an conserved SQE motif, which is rapidly phosphorylated at sites of double strand breaks and stalled replication forks. Phosphorylated H2AX may serve to recruit other repair proteins to the site of DNA damage. H2AX has been implicated in both homologous recombination and nonhomologous end joining DNA repair pathways.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Immunofluorescence (1:400 - 1:800)
• Western blot

Immunohistochemistry: LS-B3282 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3282 was determined to be 2.5 ug/ml.

Tris-citrate/phosphate, 0.09% sodium azide.

Store at 4°C or -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16104 NCBI: NM_002105 NP_002096.1