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| Catalog Number | Size | Price |
|---|---|---|
| LS-C7424-100 | 100 µg | $725 |
Monoclonal Mouse anti‑Human H2AFX / H2AX Antibody (phospho‑Ser139, IF, WB) LS‑C7424
Monoclonal Mouse anti‑Human H2AFX / H2AX Antibody (phospho‑Ser139, IF, WB) LS‑C7424
Antibody:
H2AFX / H2AX Mouse anti-Human Monoclonal (pSer139) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human, Monkey, Bovine, Dog, Rabbit
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
H2AFX / H2AX Mouse anti-Human Monoclonal (pSer139) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human, Monkey, Bovine, Dog, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
H2AX antibody LS-C7424 is an unconjugated mouse monoclonal antibody to H2AX (H2AFX) (pSer139) from human. It is reactive with human, bovine, dog and other species. Validated for ICC, IF, IP and WB.
Target
Human H2AFX / H2AX
Synonyms
H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
Host
Mouse
Reactivity
Human, Monkey, Bovine, Dog, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide (C-KATQA[pS]QEY) corresponding to aa134-142 of human histone H2A. X. (KLH). The immunizing sequence has 8 identical amino acids in yeast and mouse.
Epitope
pSer139
Specificity
Recognizes human Histone H2A. X phosphorylated at Ser139, Mr 15kD. A broad species cross-reactivity is expected based on conservation of sequence homology.
Applications
- ICC (2 µg/ml)
- Immunofluorescence
- Western blot
- Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a IgG (HRP) GtxMo and a chemiluminescence detection system. Immunocytochemistry: 2 ug/ml detects phosphorylated histone H2A. X (Ser139) in HeLa cells treated with 0.5 uM staurosporine for 4-6 hours. Positive control: UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue.
Presentation
PBS, pH 7.6, 0.07% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About H2AFX / H2AX
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
ICC, Immunofluorescence, Western blot
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
![H2AFX / H2AX Antibody - Immunofluorescence of monoclonal antibody to H2AFX on HeLa cell . [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/h2afx-h2ax-antibody-clone-3f4-ls-c197392/145163_751806.jpg)
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
