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Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (phospho‑Ser140, IHC, IF, WB) LS‑C287018
Polyclonal Rabbit anti‑Human H2AFX / H2AX Antibody (phospho‑Ser140, IHC, IF, WB) LS‑C287018
Antibody:
H2AFX / H2AX Rabbit anti-Human Polyclonal (pSer140) Antibody
Application:
IHC, ICC, IF, WB
Format:
Unconjugated, Unmodified
Overview
Antibody:
H2AFX / H2AX Rabbit anti-Human Polyclonal (pSer140) Antibody
Application:
IHC, ICC, IF, WB
Format:
Unconjugated, Unmodified
Specifications
Description
H2AX antibody LS-C287018 is an unconjugated rabbit polyclonal antibody to H2AX (H2AFX) (pSer140) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB.
Target
Human H2AFX / H2AX
Synonyms
H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
A portion of GeneID: 3014 corresponding to amino acids surrounding serine 140 of UniprotID: P16104
Specificity
Region surrounding phosphorylated serine 140 of human histone H2AX using the numbering given in entry NP_002096.1 (GeneID 3014).
Applications
- IHC (1:500 - 1:2000)
- ICC (1:1000 - 1:5000)
- Immunofluorescence (1:500 - 1:5000)
- Western blot (1:2000 - 1:10000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF: 1:500-1:5000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
ICC, Immunofluorescence, Western blot
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human
Applications:
Immunofluorescence, ELISA
Species:
Human, Mouse
Applications:
IHC, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024