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BUB3

BUB3 mitotic checkpoint protein

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Gene Name: BUB3 mitotic checkpoint protein
Synonyms: BUB3, Budding uninhibited by benomyl, HBUB3, BUB3L, Mitotic checkpoint component
Target Sequences: NM_004725 NP_004716.1 O43684

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Products
Antibodies (29)
Primary (29)
BUB3 (29)
Human (28)
Mouse (16)
Rat (13)
Bovine (4)
Chimpanzee (3)
Drosophila melanogaster (1)
Guinea pig (4)
Hamster (3)
Horse (4)
Monkey (5)
Orangutan (3)
Pig (4)
Rabbit (4)
Sheep (1)
IHC (12)
IHC-Fr (1)
IHC-P (5)
WB (24)
ELISA (12)
ICC (3)
IF (8)
IP (1)
Peptide-ELISA (2)
rabbit (27)
mouse (1)
goat (1)
Product Group
IHCPlus (2)
Ig (1)
IgG (17)
IgG2b,k (1)
monoclonal mc (1)
polyclonal pc (28)
AT2H6 (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (23)
N-Terminus (5)
aa275-325 (2)
260-340 aa, C-terminal (1)
C-Terminus (1)
His274 (1)
aa 201-230 (1)
aa1-328 (1)
aa1-50 (1)
aa279-328 (1)
aa76-90 (1)
No (29)
BUB3 Antibody - Anti-BUB3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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BUB3 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, ICC, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$460
BUB3 Antibody - Western blot analysis of lysate from fruit fly, using BUB3 Antibody (Drosophila). BUB3 Antibody (Drosophila) was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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BUB3 Rabbit anti-Drosophila melanogaster Polyclonal (aa 201-230) Antibody
Drosophila melanogaster
ELISA, WB
Unconjugated
400 µl/$393
BUB3 Antibody - Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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BUB3 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human, Monkey
ICC, IF, IHC, IHC-P, IP, WB
Unconjugated
100 µl/$299
BUB3 Antibody - Anti-BUB3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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BUB3 Rabbit anti-Human Polyclonal Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
BUB3 Antibody - BUB3 antibody. IHC(P): Rat Testis Tissue.
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BUB3 Rabbit anti-Human Polyclonal (aa76-90) Antibody
Rabbit, Mouse, Guinea pig, Sheep, Bovine, Rat, Pig, Horse, Human, Monkey
ICC, IHC, IHC-Fr, IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
BUB3 Antibody - Western blot of Bub3 antibody
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BUB3 Rabbit anti-Human Polyclonal (260-340 aa, C-terminal) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
BUB3 Antibody - IHC of Bub3 (H274) pAb in paraffin-embedded human brain tissue.
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BUB3 Rabbit anti-Human Polyclonal (His274) Antibody
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
50 µl/$321; 100 µl/$403
BUB3 Antibody - Immunohistochemistry of paraffin-embedded Rat kidney tissue.
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BUB3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
BUB3 Antibody - Immunohistochemistry of paraffin-embedded human prostate cancer tissue using BUB3 Antibody at dilution of 1:100
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BUB3 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC, WB
Unconjugated
50 µg/$294; 100 µg/$360
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BUB3 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
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BUB3 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
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BUB3 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100 µl/$332
BUB3 Antibody - Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:1000 (1 ug/ml).
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BUB3 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Human
IHC, WB
Unconjugated
20 µl/$295; 100 µl/$414
BUB3 Antibody - Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:1000 (1 ug/ml).
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BUB3 Rabbit anti-Human Polyclonal (aa275-325) Antibody
Human
IHC, WB
Unconjugated
10 µl/$295; 100 µl/$520
BUB3 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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BUB3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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BUB3 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF
Unconjugated
100 µg/$341
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BUB3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
BUB3 Antibody - The cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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BUB3 Mouse anti-Human Monoclonal (AT2H6) Antibody
Human
ELISA, WB
Unconjugated
50 µl/$303; 100 µl/$365
BUB3 Antibody - Western blot analysis of extracts from HeLa cells, Jurkat cells and 293 cells, using BUB3 antibody.
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BUB3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
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BUB3 Goat anti-Human Polyclonal (aa1-328) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$941
BUB3 Antibody - Western blot analysis of lysates from HeLa cells, Jurkat cells, and 293 cells, using BUB3 Antibody. The lane on the right is blocked with the synthesized peptide.
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BUB3 Rabbit anti-Human Polyclonal (aa279-328) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
BUB3 Antibody - Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:250.
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BUB3 Rabbit anti-Human Polyclonal (aa275-325) Antibody
Human
IHC
Unconjugated
2.5 µg/$295; 100 µl/$532
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BUB3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
50 µg/$305; 100 µg/$357
BUB3 Antibody - BUB3 antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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BUB3 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Mouse, Human
WB
Unconjugated
100 µl/$424
BUB3 Antibody - All lanes : Anti-BUB3 Antibody at 1:1000 dilution Lane 1: HeLa whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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BUB3 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
100 µl/$386
Viewing 1-25 of 29 product results


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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).