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Vector Laboratories, Inc.
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Customer Service: (800) 227-6666 / (650) 697-3600


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Catalog Number Size Price
LS-C801366-100 100 µl (1 mg/ml) $379 
LS-C801366-200 200 µl (1 mg/ml) $421 
BUB3 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
BUB3 Antibody - Western blot analysis of BUB3 antibody expression in 293T cells lysates.
BUB3 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
BUB3 Antibody - Western blot analysis of BUB3 antibody expression in 293T cells lysates.
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Polyclonal Rabbit anti‑Human BUB3 Antibody (IF, WB) LS‑C801366

Polyclonal Rabbit anti‑Human BUB3 Antibody (IF, WB) LS‑C801366

Antibody:
BUB3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801366-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
BUB3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
BUB3 antibody LS-C801366 is an unconjugated rabbit polyclonal antibody to BUB3 from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human BUB3
Synonyms
BUB3 | Budding uninhibited by benomyl | HBUB3 | BUB3L | Mitotic checkpoint component
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
Synthesized peptide derived from human Bub3
Specificity
BUB3 Antibody detects endogenous levels of total BUB3.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BUB3
O43684 NM_004725 NP_004716.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/4/2024