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Catalog Number Size Price
LS-C312796-10 10 µg $318 
LS-C312796-100 100 µg (0.5 mg/ml) $470 
BUB3 Antibody - IHC analysis of BUB3 using anti-BUB3 antibody. BUB3 was detected in frozen section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BUB3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BUB3 Antibody - BUB3 antibody. IHC(P): Human Breast Cancer Tissue.
BUB3 Antibody - BUB3 antibody. IHC(P): Rat Testis Tissue.
BUB3 Antibody - BUB3 antibody. ICC: HELA Cell.
BUB3 Antibody - BUB3 antibody. ICC: A549 Cell.
BUB3 Antibody - Anti-Bub3 antibody, Western blotting All lanes: Anti Bub3 at 0.5ug/ml Lane 1: HELA Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 37KD Observed bind size: 37KD
BUB3 Antibody - WB of BUB3 antibody. Lane 1: HELA Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4:COLO320 Cell Lysate.
BUB3 Antibody - IHC analysis of BUB3 using anti-BUB3 antibody. BUB3 was detected in frozen section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BUB3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BUB3 Antibody - BUB3 antibody. IHC(P): Human Breast Cancer Tissue.
BUB3 Antibody - BUB3 antibody. IHC(P): Rat Testis Tissue.
BUB3 Antibody - BUB3 antibody. ICC: HELA Cell.
BUB3 Antibody - BUB3 antibody. ICC: A549 Cell.
BUB3 Antibody - Anti-Bub3 antibody, Western blotting All lanes: Anti Bub3 at 0.5ug/ml Lane 1: HELA Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 37KD Observed bind size: 37KD
BUB3 Antibody - WB of BUB3 antibody. Lane 1: HELA Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4:COLO320 Cell Lysate.
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Polyclonal Rabbit anti‑Human BUB3 Antibody (aa76‑90, IHC, WB) LS‑C312796

Polyclonal Rabbit anti‑Human BUB3 Antibody (aa76‑90, IHC, WB) LS‑C312796

Note: This antibody replaces LS-C388071
Antibody:
BUB3 Rabbit anti-Human Polyclonal (aa76-90) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312796-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
BUB3 Rabbit anti-Human Polyclonal (aa76-90) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
BUB3 antibody LS-C312796 is an unconjugated rabbit polyclonal antibody to BUB3 (aa76-90) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IHC and WB.
Target
Human BUB3
Synonyms
BUB3 | Budding uninhibited by benomyl | HBUB3 | BUB3L | Mitotic checkpoint component
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Bub3(76-90 aa DHQLKMHDLNTDQEN), identical to the related rat and mouse sequences.
Epitope
aa76-90
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BUB3
O43684 NM_004725 NP_004716.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/4/2024