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Catalog Number Size Price
LS-C353144-100 100 µl (1 mg/ml) $299 
BUB3 Antibody - Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
BUB3 Antibody - Immunofluorescent analysis of BUB3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BUB3 Antibody - Western blot analysis of BUB3 expression in HeLa (A); HEK293T (B) whole cell lysates.
BUB3 Antibody - Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
BUB3 Antibody - Immunofluorescent analysis of BUB3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
BUB3 Antibody - Western blot analysis of BUB3 expression in HeLa (A); HEK293T (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human BUB3 Antibody (C‑Terminus, IHC, IF, WB) LS‑C353144

Polyclonal Rabbit anti‑Human BUB3 Antibody (C‑Terminus, IHC, IF, WB) LS‑C353144

Antibody:
BUB3 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C353144-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
BUB3 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
BUB3 antibody LS-C353144 is an unconjugated rabbit polyclonal antibody to BUB3 (C-Terminus) from human. It is reactive with human and monkey. Validated for ICC, IF, IHC, IP and WB.
Target
Human BUB3
Synonyms
BUB3 | Budding uninhibited by benomyl | HBUB3 | BUB3L | Mitotic checkpoint component
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human BUB3.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of BUB3 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BUB3
O43684 NM_004725 NP_004716.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 12/4/2024