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Catalog Number	Size	Price
LS-B16419-50	50 µl (1 mg/ml)	$460

Vimentin Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Vimentin Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Vimentin Antibody - Immunofluorescent analysis of Vimentin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Vimentin Antibody - Western blot analysis of Vimentin expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates.

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PathPlus™ Monoclonal Mouse anti‑Human Vimentin Antibody (IHC, IF, WB) LS‑B16419

Name: PathPlus™ Vimentin Monoclonal Antibody Mouse anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B16419-50
Price: 460.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

Cancer Pathology Cancer

(5.0) Rating

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Note: This antibody replaces LS-C413598

Antibody:

Vimentin Mouse anti-Human Monoclonal Antibody

Application:

IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$460

LS-B16419-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

Vimentin Mouse anti-Human Monoclonal Antibody

Application:

IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

Vimentin (VIM) is an intermediate filament component of the cytoskeleton of mesenchymal cells. It is important for maintaining cell integrity and shape. Vimentin also regulates cell migration via cytoskeletal rearrangements and is involved in regulating autophagy. In immunohistochemistry, it is positive in the cytoplasm of cells of mesenchymal origin, such as lymphocytes, endothelial cells, astrocytes, epidermal Langerhans cells, macrophages, and interstitial stromal cells. In cancer, it is positive in lymphomas and stromal tumors and is helpful for identifying melanomas, schwannomas and tumors of mesenchymal origin. References: Am J Surg Pathol. 2011;35:1722, PMID: 21997693; F1000Res. 2018 Nov 15;7. pii: F1000 Faculty Rev-1796, PMID: 30505430; PLoS One. 2019 Jan 30;14(1):e0209665, PMID: 30699149; Toxicol Pathol. 2010 Jan;38(1):131-41, PMID: 20028992

Target

Human Vimentin

Synonyms

VIM | Vimentin | vimenin

See All Vimentin Antibodies

Host

Mouse

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG1 Monoclonal

Conjugations

Unconjugated

Purification

Purified

Modifications

Unmodified

Immunogen

Recombinant protein corresponding to human Vimentin.

Specificity

Recognizes endogenous levels of Vimentin protein.

Applications

IHC - Paraffin (10 µg/ml)
ICC (1:100 - 1:200)
Immunofluorescence (1:100 - 1:200)
Western blot (1:1000 - 1:3000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About Vimentin

Uniprot: P08670 NCBI: NM_003380 NP_003371.2