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| Catalog Number | Size | Price |
|---|---|---|
| LS-B7135-50 | 50 µl | $460 |
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2 of 2
PathPlus™ Monoclonal Mouse anti‑Human Vimentin Antibody (clone 35E2, IHC, WB) LS‑B7135
PathPlus™ Monoclonal Mouse anti‑Human Vimentin Antibody (clone 35E2, IHC, WB) LS‑B7135
Note: This antibody replaces LS-C62127
Antibody:
Vimentin Mouse anti-Human Monoclonal (35E2) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Vimentin Mouse anti-Human Monoclonal (35E2) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
Vimentin (VIM) is an intermediate filament component of the cytoskeleton of mesenchymal cells. It is important for maintaining cell integrity and shape. Vimentin also regulates cell migration via cytoskeletal rearrangements and is involved in regulating autophagy. In immunohistochemistry, it is positive in the cytoplasm of cells of mesenchymal origin, such as lymphocytes, endothelial cells, astrocytes, epidermal Langerhans cells, macrophages, and interstitial stromal cells. In cancer, it is positive in lymphomas and stromal tumors and is helpful for identifying melanomas, schwannomas and tumors of mesenchymal origin.
References: Am J Surg Pathol. 2011;35:1722, PMID: 21997693; F1000Res. 2018 Nov 15;7. pii: F1000 Faculty Rev-1796, PMID: 30505430; PLoS One. 2019 Jan 30;14(1):e0209665, PMID: 30699149; Toxicol Pathol. 2010 Jan;38(1):131-41, PMID: 20028992
Host
Mouse
Reactivity
Human, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
35E2
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Recombinant human protein purified from E. coli (His-Vimentin).
Applications
- IHC
- IHC - Paraffin (1:400)
- Western blot (1:2000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Presentation
HEPES, 0.15 M NaCl, 0.03% Sodium Azide, 50% Glycerol, 0.01% BSA
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About Vimentin
Validation
Anti-Vimentin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:400.
Anti-Vimentin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:400.
See More About...
LSBio Ratings
PathPlus™ Vimentin Antibody (clone 35E2) for IHC, WB/Western LS-B7135 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Bovine, Human, Monkey, Mouse, Rat, Dog, Goat, Hamster, Pig, Chicken
Applications:
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
