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| Catalog Number | Size | Price |
|---|---|---|
| LS-B4669-50 | 50 µl (0.5 mg/ml) | $460 |
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PathPlus™ Polyclonal Rabbit anti‑Human Vimentin Antibody (C‑Terminus, IHC, WB) LS‑B4669
PathPlus™ Polyclonal Rabbit anti‑Human Vimentin Antibody (C‑Terminus, IHC, WB) LS‑B4669
Note: This antibody replaces LS-C81189
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
Vimentin (VIM) is an intermediate filament component of the cytoskeleton of mesenchymal cells. It is important for maintaining cell integrity and shape. Vimentin also regulates cell migration via cytoskeletal rearrangements and is involved in regulating autophagy. In immunohistochemistry, it is positive in the cytoplasm of cells of mesenchymal origin, such as lymphocytes, endothelial cells, astrocytes, epidermal Langerhans cells, macrophages, and interstitial stromal cells. In cancer, it is positive in lymphomas and stromal tumors and is helpful for identifying melanomas, schwannomas and tumors of mesenchymal origin.
References: Am J Surg Pathol. 2011;35:1722, PMID: 21997693; F1000Res. 2018 Nov 15;7. pii: F1000 Faculty Rev-1796, PMID: 30505430; PLoS One. 2019 Jan 30;14(1):e0209665, PMID: 30699149; Toxicol Pathol. 2010 Jan;38(1):131-41, PMID: 20028992
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from C-Terminus of human VIM (P08670, NP_003371). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%); Lizard, Xenopus (92%); Bat (86%); Stickleback (84%).
Epitope
C-Terminus
Specificity
Human VIM / Vimentin
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About Vimentin
Validation
Anti-Vimentin antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Vimentin antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Vimentin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Vimentin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
PathPlus™ Vimentin Antibody (C-Terminus) for IHC, WB/Western LS-B4669 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Bovine, Human, Monkey, Mouse, Rat, Dog, Goat, Hamster, Pig, Chicken
Applications:
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
