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Catalog Number	Size	Price
LS-B16696-50	50 µg (0.5 mg/ml)	$460

Vimentin Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

Vimentin Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Vimentin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Vimentin Antibody - Vimentin antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/Intermediate filament staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Vimentin Antibody - Vimentin antibody (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Vimentin Antibody - Vimentin antibody (0.1µg/ml) staining of Mouse (A) and (2µg/ml) Rat (B) Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Vimentin Antibody - Vimentin antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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PathPlus™ Polyclonal Goat anti‑Human Vimentin Antibody (IHC, IF, WB) LS‑B16696

Name: PathPlus™ Vimentin Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B16696-50
Price: 460.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

Cancer Pathology

(5.0) Rating

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Note: This antibody replaces LS-C502294

Antibody:

Vimentin Goat anti-Human Polyclonal Antibody

Application:

IHC-P, IF, WB, Flo, Peptide-ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$460

LS-B16696-50

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

Vimentin Goat anti-Human Polyclonal Antibody

Application:

IHC-P, IF, WB, Flo, Peptide-ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

Vimentin (VIM) is an intermediate filament component of the cytoskeleton of mesenchymal cells. It is important for maintaining cell integrity and shape. Vimentin also regulates cell migration via cytoskeletal rearrangements and is involved in regulating autophagy. In immunohistochemistry, it is positive in the cytoplasm of cells of mesenchymal origin, such as lymphocytes, endothelial cells, astrocytes, epidermal Langerhans cells, macrophages, and interstitial stromal cells. In cancer, it is positive in lymphomas and stromal tumors and is helpful for identifying melanomas, schwannomas and tumors of mesenchymal origin. References: Am J Surg Pathol. 2011;35:1722, PMID: 21997693; F1000Res. 2018 Nov 15;7. pii: F1000 Faculty Rev-1796, PMID: 30505430; PLoS One. 2019 Jan 30;14(1):e0209665, PMID: 30699149; Toxicol Pathol. 2010 Jan;38(1):131-41, PMID: 20028992

Target

Human Vimentin

Synonyms

VIM | Vimentin | vimenin

See All Vimentin Antibodies

Host

Goat

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-QVINETSQHHDDLE, from the C-Terminus of protein sequence according to NP_003371.2.

Applications

IHC - Paraffin (5 µg/ml)
Immunofluorescence (5 µg/ml)
Western blot (0.1 - 2 µg/ml)
Flow Cytometry (10 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:4000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About Vimentin

Uniprot: P08670 NCBI: NM_003380 NP_003371.2