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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16228-100 | 100 µg (0.2 mg/ml) | $525 |
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PathPlus™ Recombinant Monoclonal Rabbit anti‑Human PLAP / Alkaline Phosphatase Antibody (clone ALPP/2899R, IHC) LS‑B16228
PathPlus™ Recombinant Monoclonal Rabbit anti‑Human PLAP / Alkaline Phosphatase Antibody (clone ALPP/2899R, IHC) LS‑B16228
Note: This antibody replaces LS-C751149
Antibody:
PLAP / Alkaline Phosphatase Rabbit anti-Human Recombinant Monoclonal (ALPP/2899R) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
PLAP / Alkaline Phosphatase Rabbit anti-Human Recombinant Monoclonal (ALPP/2899R) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
PLAP (alkaline phosphatase, placental type, ALPP) is an allosteric enzyme expressed in the placenta. PLAP is a membrane-bound protein that functions to catalyze phosphate monoester hydrolysis and transphosphorylation. In immunohistochemistry of cancer, PLAP is marker used to identify seminoma and malignant germ cell tumors (embryonal carcinoma and dysgerminoma), and it is also positive in ovarian, breast, lung, stomach, and pancreatic carcinomas. In normal tissues, PLAP stains placental trophoblasts.
References: J Phys Chem B. 2014 Dec 11;118(49):14302-13, PMID: 25409280; J Lab Med. 2004. 28 (2): 109–115, DOI:10.1515/labmed.2004.018; Clin. Biochem. 20 (6): 387–92, PMID: 3325192.
Target
Human PLAP / Alkaline Phosphatase
Synonyms
ALPP | ALP | Alkaline phosphomonoesterase | Glycerophosphatase | PALP | PLAP | PLAP-1 | Alp1 | Germ-cell alkaline phosphatase | Nagao isozyme | Regan isozyme
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Recombinant Monoclonal
Clone
ALPP/2899R
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified.
Also available Azide-free, Carrier-free.
Immunogen
Recombinant full-length human ALPP protein. Antigen Molecular Weight: 70 kDa
Specificity
Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
Applications
- IHC - Paraffin (1:100)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistology (Formalin-fixed - 0.25-0.5 ug/ml for 30 minutes at RT - No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.. Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PLAP / Alkaline Phosphatase
Validation
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ PLAP / Alkaline Phosphatase Antibody (clone ALPP/2899R) for IHC LS-B16228 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Frozen, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Frozen, Western blot, ELISA
Species:
Human
Applications:
IHC, Western blot, ELISA
Species:
Human
Applications:
Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
