Target
Human SIRT1 / Sirtuin 1
Synonyms
SIRT1 | SIR2-like protein 1 | SIR2alpha | SIR2L1 | Sirtuin 1 | Sir2-like 1 | Sirtuin type 1 | HSIR2 | HSIRT1
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen
A synthesized peptide derived from human SirT1 around the phosphorylation site of Serine 47.
Specificity
Phospho-SirT1 (Ser47) Antibody detects endogenous levels of SirT1 only when phosphorylated at Serine 47.
Applications
- Western blot (1:500 - 1:2000)
- Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee