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| Catalog Number | Size | Price |
|---|---|---|
| LS-B8356-50 | 50 µg (0.5 mg/ml) | $485 |
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2 of 2
IHC‑plus™ Polyclonal Goat anti‑Human SIRT1 / Sirtuin 1 Antibody (aa577‑590, IHC, WB) LS‑B8356
IHC‑plus™ Polyclonal Goat anti‑Human SIRT1 / Sirtuin 1 Antibody (aa577‑590, IHC, WB) LS‑B8356
Note: This antibody replaces LS-C139765
Antibody:
SIRT1 / Sirtuin 1 Goat anti-Human Polyclonal (aa577-590) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
SIRT1 / Sirtuin 1 Goat anti-Human Polyclonal (aa577-590) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
Sirtuin 1 antibody LS-B8356 is an unconjugated goat polyclonal antibody to Sirtuin 1 (SIRT1) (aa577-590) from human. It is reactive with human and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human SIRT1 / Sirtuin 1
Synonyms
SIRT1 | SIR2-like protein 1 | SIR2alpha | SIR2L1 | Sirtuin 1 | Sir2-like 1 | Sirtuin type 1 | HSIR2 | HSIRT1
Host
Goat
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-EKPQEVQTSRNVES, from the internal region of the protein sequence according to NP_036370.2NP_001135970.1.
Epitope
aa577-590
Specificity
Human SIRT1. This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1).
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.05 - 0.2 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 120kD band observed in lysates of cell line Jurkat (calculated MW of 81.7kD according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9.; PMID: 12960381). Recommended concentration: 0.05-0.2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SIRT1 / Sirtuin 1
Validation
Anti-SIRT1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-SIRT1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ SIRT1 / Sirtuin 1 Antibody (aa577-590) for IHC, WB/Western LS-B8356 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Dysregulated miR-34a-SIRT1-acetyl p65 axis is a potential mediator of immune activation in the colon during chronic simian immunodeficiency virus infection of rhesus macaques. Mohan M, Kumar V, Lackner AA, Alvarez X. Journal of immunology (Baltimore, Md. : 1950). 2015 194:291-306. (IF; Rhesus monkey)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Reactivity:
Human
Range:
78-5000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/15/2024
Date Requested: 11/15/2024
