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Catalog Number	Size	Price
LS-C746926-20	20 µl	$275
LS-C746926-100	100 µl	$389

SIRT1 / Sirtuin 1 Antibody - Immunohistochemistry of paraffin-embedded rat testis using SIRT1 antibody at dilution of 1:100 (40x lens).

SIRT1 / Sirtuin 1 Antibody - Immunohistochemistry of paraffin-embedded human colon carcinoma using SIRT1 antibody at dilution of 1:100 (40x lens).

SIRT1 / Sirtuin 1 Antibody - Immunohistochemistry of paraffin-embedded mouse testis using SIRT1 antibody at dilution of 1:100 (40x lens).

SIRT1 / Sirtuin 1 Antibody - Immunohistochemistry of paraffin-embedded rat liver using SIRT1 antibody at dilution of 1:100 (40x lens).

SIRT1 / Sirtuin 1 Antibody - Immunofluorescence analysis of HeLa cells using SIRT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

SIRT1 / Sirtuin 1 Antibody - Western blot analysis of extracts from normal (control) and SIRT1 knockout (KO) HeLa cells, using SIRT1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.

SIRT1 / Sirtuin 1 Antibody - Western blot analysis of extracts of various cell lines, using SIRT1 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.

SIRT1 / Sirtuin 1 Antibody - Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug SIRT1 antibody. Western blot was performed from the immunoprecipitate using SIRT1 antibody at a dilition of 1:1000.

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Polyclonal Rabbit anti‑Human SIRT1 / Sirtuin 1 Antibody (IHC, IF, WB) LS‑C746926

Name: Anti-SIRT1 / Sirtuin 1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C746926-20
Price: 275.0000 USD
Availability: InStock

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Antibody:

SIRT1 / Sirtuin 1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, IP

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$275

LS-C746926-20

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

SIRT1 / Sirtuin 1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, IP

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

Sirtuin 1 antibody LS-C746926 is an unconjugated rabbit polyclonal antibody to Sirtuin 1 (SIRT1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP and WB.

Target

Human SIRT1 / Sirtuin 1

Synonyms

See All SIRT1 / Sirtuin 1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2). VALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS

Applications

IHC (1:50 - 1:200)
Immunofluorescence (1:50 - 1:200)
Western blot (1:500 - 1:2000)
Immunoprecipitation (1:50 - 1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

The predicted MW is 61kDa/81kDa, while the observed MW by Western blot was 130kDa.

Presentation

PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About SIRT1 / Sirtuin 1