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Catalog Number Size Price
LS-C359279-0.1 0.1 ml (1 mg/ml) $386 
SIRT1 / Sirtuin 1 Antibody - Immunohistochemistry-Paraffin: SIRT1 Antibody (1F3) - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.
SIRT1 / Sirtuin 1 Antibody - Immunocytochemistry/Immunofluorescence: SIRT1 Antibody (1F3) - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
SIRT1 / Sirtuin 1 Antibody - Western Blot: SIRT1 Antibody (1F3) - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), HeLa (3), HEK293 (4) and A549 (5) cell lysates.
SIRT1 / Sirtuin 1 Antibody - Flow Cytometry: SIRT1 Antibody (1F3) - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).
SIRT1 / Sirtuin 1 Antibody - Flow Cytometry: SIRT1 Antibody (1F3) - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
SIRT1 / Sirtuin 1 Antibody - Immunohistochemistry-Paraffin: SIRT1 Antibody (1F3) - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.
SIRT1 / Sirtuin 1 Antibody - Immunocytochemistry/Immunofluorescence: SIRT1 Antibody (1F3) - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
SIRT1 / Sirtuin 1 Antibody - Western Blot: SIRT1 Antibody (1F3) - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), HeLa (3), HEK293 (4) and A549 (5) cell lysates.
SIRT1 / Sirtuin 1 Antibody - Flow Cytometry: SIRT1 Antibody (1F3) - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).
SIRT1 / Sirtuin 1 Antibody - Flow Cytometry: SIRT1 Antibody (1F3) - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Monoclonal Mouse anti‑Human SIRT1 / Sirtuin 1 Antibody (clone 1F3, IHC, IF, WB) LS‑C359279

Monoclonal Mouse anti‑Human SIRT1 / Sirtuin 1 Antibody (clone 1F3, IHC, IF, WB) LS‑C359279

Antibody:
SIRT1 / Sirtuin 1 Mouse anti-Human Monoclonal (1F3) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Primate
Format:
Unconjugated, Unmodified
Price
Catalog Number
$386
LS-C359279-0.1
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SIRT1 / Sirtuin 1 Mouse anti-Human Monoclonal (1F3) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Primate
Format:
Unconjugated, Unmodified

Specifications

Description
Sirtuin 1 antibody LS-C359279 is an unconjugated mouse monoclonal antibody to Sirtuin 1 (SIRT1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IF, IHC and WB.
Target
Human SIRT1 / Sirtuin 1
Synonyms
SIRT1 | SIR2-like protein 1 | SIR2alpha | SIR2L1 | Sirtuin 1 | Sir2-like 1 | Sirtuin type 1 | HSIR2 | HSIRT1
Host
Mouse
Reactivity
Human, Mouse, Rat, Primate (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
1F3
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6]
Specificity
Human SIRT1 / Sirtuin 1. Human SIRT1 / Sirtuin 1
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • ICC (1:10 - 1:1000)
  • Immunofluorescence (1:10 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1 µg/10E6 cells)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This SIRT1 (1F3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.
Presentation
PBS, 0.03% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIRT1 / Sirtuin 1
Q96EB6 NM_012238 NP_036370.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/15/2024