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Catalog Number Size Price
LS-C792239-100 100 µg $511 
SIRT1 / Sirtuin 1 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SIRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:2000)
SIRT1 / Sirtuin 1 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SIRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:2000)
SIRT1 / Sirtuin 1 Antibody - Western blot analysis of extracts. (35ug) from 3 different cell lines by using anti-SIRT1 monoclonal antibody. (1:500)
SIRT1 / Sirtuin 1 Antibody - Equivalent amounts of cell lysates  and SIRT1-Knockout Hela cells  were separated by SDS-PAGE and immunoblotted with anti-SIRT1 monoclonal antibodyThen the blotted membrane was stripped and reprobed with anti-b-actin antibody  as a loading control. (1:500)
SIRT1 / Sirtuin 1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY SIRT1. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT1. (1:2000)
SIRT1 / Sirtuin 1 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SIRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:2000)
SIRT1 / Sirtuin 1 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SIRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 120°C for 3 min. (1:2000)
SIRT1 / Sirtuin 1 Antibody - Western blot analysis of extracts. (35ug) from 3 different cell lines by using anti-SIRT1 monoclonal antibody. (1:500)
SIRT1 / Sirtuin 1 Antibody - Equivalent amounts of cell lysates  and SIRT1-Knockout Hela cells  were separated by SDS-PAGE and immunoblotted with anti-SIRT1 monoclonal antibodyThen the blotted membrane was stripped and reprobed with anti-b-actin antibody  as a loading control. (1:500)
SIRT1 / Sirtuin 1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY SIRT1. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT1. (1:2000)
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Monoclonal Mouse anti‑Human SIRT1 / Sirtuin 1 Antibody (clone OTI5B2, Carrier‑free, aa552‑676, IHC, WB) LS‑C792239

Monoclonal Mouse anti‑Human SIRT1 / Sirtuin 1 Antibody (clone OTI5B2, Carrier‑free, aa552‑676, IHC, WB) LS‑C792239

Antibody:
SIRT1 / Sirtuin 1 Mouse anti-Human Monoclonal (aa552-676) (Carrier-free) (OTI5B2) Antibody
Application:
IHC, WB
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$511
LS-C792239-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SIRT1 / Sirtuin 1 Mouse anti-Human Monoclonal (aa552-676) (Carrier-free) (OTI5B2) Antibody
Application:
IHC, WB
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
Sirtuin 1 antibody LS-C792239 is an unconjugated mouse monoclonal antibody to human Sirtuin 1 (SIRT1) (aa552-676). Validated for IHC and WB.
Target
Human SIRT1 / Sirtuin 1
Synonyms
SIRT1 | SIR2-like protein 1 | SIR2alpha | SIR2L1 | Sirtuin 1 | Sir2-like 1 | Sirtuin type 1 | HSIR2 | HSIRT1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
OTI5B2
Conjugations
Unconjugated
Purification
Purified from ascites.
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Human recombinant protein fragment corresponding to amino acids 552-676 of human SIRT1 (NP_036370) produced in E.coli.
Epitope
aa552-676
Applications
  • IHC (1:2000)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIRT1 / Sirtuin 1
Q96EB6 NM_012238 NP_036370.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/16/2024