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Antibodies > PPARG / PPAR Gamma > LS-C368478
 
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Catalog Number Size Price
LS-C368478-30 30 µl (1 mg/ml) $255 
LS-C368478-100 100 µl (1 mg/ml) $299 
LS-C368478-200 200 µl (1 mg/ml) $351 
PPARG / PPAR Gamma Antibody - Immunohistochemical analysis of PPAR gamma staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PPARG / PPAR Gamma Antibody - Immunofluorescent analysis of PPAR gamma staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
PPARG / PPAR Gamma Antibody - Western blot analysis of PPAR gamma expression in HeLa (A); MCF7 (B); mouse brain (C) whole cell lysates.
PPARG / PPAR Gamma Antibody - Immunohistochemical analysis of PPAR gamma staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PPARG / PPAR Gamma Antibody - Immunofluorescent analysis of PPAR gamma staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
PPARG / PPAR Gamma Antibody - Western blot analysis of PPAR gamma expression in HeLa (A); MCF7 (B); mouse brain (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human PPARG / PPAR Gamma Antibody (Internal, IHC, IF, WB) LS‑C368478

Polyclonal Rabbit anti‑Human PPARG / PPAR Gamma Antibody (Internal, IHC, IF, WB) LS‑C368478

Submit Review LSBio Guarantee
Antibody:
PPARG / PPAR Gamma Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Dog, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C368478-30
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PPARG / PPAR Gamma Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Dog, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
PPAR Gamma antibody LS-C368478 is an unconjugated rabbit polyclonal antibody to PPAR Gamma (PPARG) (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human PPARG / PPAR Gamma
Synonyms
PPARG | NR1C3 | PPAR gamma | PPAR gamma 2 | PPARG1 | PPARG2 | PPARgamma | CIMT1 | GLM1 | PPAR-gamma
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human PPAR gamma.
Epitope
Internal
Specificity
Recognizes endogenous levels of PPAR gamma protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPARG / PPAR Gamma
P37231 NM_015869 NP_056953.2

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Featured Products

PPARG / PPAR Gamma Antibody - Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ PPARG / PPAR Gamma Antibody LS‑B130
Species: Human, Macaque
Applications: IHC, IHC - Paraffin, Western blot, ELISA
PPARG / PPAR Gamma Antibody - ELISA results of purified Rabbit anti-PPAR gamma pS273 tested against BSA-conjugated non-phospho and phospho forms of immunizing peptide. Each well was coated in duplicate with either 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate
PPARG / PPAR Gamma Antibody (phospho‑Ser273) LS‑C209422
Species: Human
Applications: IHC, Western blot, ELISA
PPARG / PPAR Gamma Antibody - Anti-PPARG antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ PPARG / PPAR Gamma Antibody LS‑B651
Species: Human, Orangutan, Mouse, Rat, Dog, Guinea pig, Hamster, Mink, Rabbit, Boar, Duck, Macaque, Squirrel
Applications: IHC, IHC - Paraffin, Western blot, ELISA
PPARG / PPAR Gamma Antibody - PPARG Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PPARG / PPAR Gamma Antibody (aa1‑30) LS‑C163891
Species: Human
Applications: Western blot, Flow Cytometry
PPARG / PPAR Gamma Antibody - This image was taken for the unconjugated form of this product. Other forms have not been tested.
PPARG / PPAR Gamma Antibody (C‑Terminus) LS‑C169542
Species: Rat
Applications: Western blot
PPARG / PPAR Gamma Antibody - Anti-PPARG antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ PPARG / PPAR Gamma Antibody (aa1‑16) LS‑B2818
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, ICC, Western blot, Gel shift
See all PPARG / PPAR Gamma Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/30/2024
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