Human PPARG / PPAR Gamma

Rabbit

Human, Monkey (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide corresponding to aa1-16 (MGETLGDSPIDPESDS) of human PPARg2 (peroxisome proliferator activated receptor g2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%); Gorilla, Cat, Bat, Dog (94%); Pig (88%); Elephant (81%).

aa1-16

Recognizes mouse PPARg2. Does not cross-react with PPARa or PPARd.

• IHC
• IHC - Paraffin (2.5 - 5 µg/ml)
• ICC (1:500)
• Western blot (1:500)
• Gel shift

Immunohistochemistry: LS-B2818 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2818 was determined to be 2.5-5 ug/ml. Western Blot: 1:500. Detects a band of ~56kD.

PBS, 1 mg/ml BSA, 0.05% Sodium Azide

Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P37231 NCBI: NM_015869 NP_056953.2