Human PPARG / PPAR Gamma

Rabbit

Human, Orangutan, Mouse, Rat, Dog, Guinea pig, Hamster, Mink, Rabbit, Boar, Duck, Macaque, Squirrel (tested or 100% immunogen sequence identity)

Human, Orangutan, Mouse, Rat, Dog, Guinea pig, Hamster, Mink, Rabbit, Boar, Duck, Macaque, Squirrel (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 250-300 of human PPAR gamma isoform 1.

A BLAST analysis was used to suggest broad reactivity with this protein from several sources. Expect reactivity against human (g1 and g2), mouse (g1 and g2), rat, squirrel, orangutan, duck (g1), macaque (g1-g7), boar (g1a-1d), mink, guinea pig, rabbit (g1 and g3), dog and hamster sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR gamma homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot
• ELISA (1:8000 - 1:32000)

Immunohistochemistry: LS-B651 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B651 was determined to be 5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P37231 NCBI: NM_015869 NP_056953.2