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Catalog Number Size Price
LS-C209422-50 50 µg (0.85 mg/ml) $567 
PPARG / PPAR Gamma Antibody - ELISA results of purified Rabbit anti-PPAR gamma pS273 tested against BSA-conjugated non-phospho and phospho forms of immunizing peptide. Each well was coated in duplicate with either 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate

Polyclonal Rabbit anti‑Human PPARG / PPAR Gamma Antibody (phospho‑Ser273, IHC, WB) LS‑C209422

Polyclonal Rabbit anti‑Human PPARG / PPAR Gamma Antibody (phospho‑Ser273, IHC, WB) LS‑C209422

Antibody:
PPARG / PPAR Gamma Rabbit anti-Human Polyclonal (pSer273) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C209422-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PPARG / PPAR Gamma Rabbit anti-Human Polyclonal (pSer273) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PPAR Gamma antibody LS-C209422 is an unconjugated rabbit polyclonal antibody to human PPAR Gamma (PPARG) (pSer273). Validated for ELISA, IHC and WB. Cited in 1 publication.
Target
Human PPARG / PPAR Gamma
Synonyms
PPARG | NR1C3 | PPAR gamma | PPAR gamma 2 | PPARG1 | PPARG2 | PPARgamma | CIMT1 | GLM1 | PPAR-gamma
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Dog, Pig, Primate (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-PPAR gamma pS273 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human PPARG protein.
Epitope
pSer273
Specificity
A BLAST analysis was used to suggest cross-reactivity with rat, mouse, primate, human, dog, bovine, and pig based on 100% sequence homology. Cross-reactivity with PPAR gamma pS273 from other sources has not been determined.
Applications
  • IHC
  • Western blot (1 µg/ml)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Anti-PPAR antibody is useful for ELISA and Western Blot. Expect a band approximately ~57kD corresponding to the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPARG / PPAR Gamma
P37231 NM_015869 NP_056953.2

LSBio Ratings

PPARG / PPAR Gamma Antibody (phospho-Ser273) for IHC, WB/Western, ELISA LS-C209422 has an LSBio Rating of
Publications (4)

Publications (1)

PPAR? Interaction with UBR5/ATMIN Promotes DNA Repair to Maintain Endothelial Homeostasis. Li CG, Mahon C, Sweeney NM, Verschueren E, Kantamani V, Li D, Hennigs JK, Marciano DP, Diebold I, Abu-Halawa O, Elliott M, Sa S, Guo F, Wang L, Cao A, Guignabert C, Sollier J, Nickel NP, Kaschwich M, Cimprich KA, Rabinovitch M. Cell reports. 2019 January;26:1333-1343.

Customer Reviews (0)


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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/30/2024