Human PODXL / Podocalyxin

Mouse

Human, Rat, Rabbit (tested or 100% immunogen sequence identity)

IgM Monoclonal

4F10

Unconjugated

PEG purified

Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.

Recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human Podocalyxin.

Extracellular Domain

Human PODXL / Podocalyxin

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. Optimal dilution of the PODXL antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00592 NCBI: NM_005397 NP_005388.2