Human PODXL / Podocalyxin

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with Biotin, FITC, HRP.

Immunoaffinity purified

Unmodified

Synthetic peptide located between aa301-350 of human PODXL (A6NHX8, NP_001018121). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey (100%); Gorilla (92%).

aa301-350

Human PODXL

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot

Immunohistochemistry: LS-B3558 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3558 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

PBS, 0.09% sodium azide, 2% sucrose

Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00592 NCBI: NM_005397 NP_005388.2