Human PODXL / Podocalyxin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived rhPodocalyxin extracellular domain.

Extracellular Domain

Recognizes human Podocalyxin. In Western Blots, this antibody does not cross-react with rmPodocalyxin or rhEndoglycan.

• ICC (10 µg/ml)
• Western blot
• Flow Cytometry

Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Immunocytochemistry: 10 ug/ml using fixed cells. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. Antibody binding may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: t 1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhPodocalyxin is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.

Lyophilized from PBS, pH 7.4, 5% Trehalose

Reconstitute in 200 ul sterile PBS

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00592 NCBI: NM_005397 NP_005388.2