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Catalog Number Size Price
LS-C815393-100 100 µl $412 
OCLN / Occludin Antibody - Immunofluorescence staining of OCLN in Hek293 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human OCLN polyclonal antibody (dilution ratio 1:1500) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane and cytoplasm.
OCLN / Occludin Antibody - Anti-OCLN rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 72 kDa.
OCLN / Occludin Antibody - Immunofluorescence staining of OCLN in Hek293 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human OCLN polyclonal antibody (dilution ratio 1:1500) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane and cytoplasm.
OCLN / Occludin Antibody - Anti-OCLN rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 72 kDa.
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Polyclonal Rabbit anti‑Human OCLN / Occludin Antibody (Preservative Free, IF, WB) LS‑C815393

Polyclonal Rabbit anti‑Human OCLN / Occludin Antibody (Preservative Free, IF, WB) LS‑C815393

Antibody:
OCLN / Occludin Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C815393-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
OCLN / Occludin Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
Occludin antibody LS-C815393 is an unconjugated rabbit polyclonal antibody to human Occludin (OCLN). Validated for ICC, IF and WB.
Target
Human OCLN / Occludin
Synonyms
OCLN | Occludin | BLCPMG
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
A synthetic peptide corresponding to the C-Terminus of Human OCLN/Occludin
Specificity
Human Occludin
Applications
  • ICC (1:1000 - 1:5000)
  • Immunofluorescence (1:1000 - 1:5000)
  • Western blot (1:500 - 1:1000)
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About OCLN / Occludin
Q16625 NM_002538 NP_002529.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024