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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2187-50 | 50 µg (0.25 mg/ml) | $485 |
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2 of 2
PathPlus™ Polyclonal Rabbit anti‑Human OCLN / Occludin Antibody (C‑Terminus, IHC, IF, WB) LS‑B2187
PathPlus™ Polyclonal Rabbit anti‑Human OCLN / Occludin Antibody (C‑Terminus, IHC, IF, WB) LS‑B2187
Note: This antibody replaces LS-C37847
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
OCLN (Occludin) is an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A GST fusion protein consisting of the C-Terminus 150 amino acids of human occludin fused to GST.
Epitope
C-Terminus
Specificity
Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.
Applications
- IHC - Paraffin (2.5 µg/ml)
- Immunofluorescence
- Western blot (1 - 3 µg/ml)
- Immunoprecipitation
- ELISA (0.1 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2187 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2187 was determined to be 2.5 ug/ml.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About OCLN / Occludin
Validation
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
PathPlus™ OCLN / Occludin Antibody (C-Terminus) for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-B2187 has an LSBio Rating of
Publications (4.3)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (4)
The tight-junction protein claudin-6 induces epithelial differentiation from mouse F9 and embryonic stem cells. Sugimoto K, Ichikawa-Tomikawa N, Satohisa S, Akashi Y, Kanai R, Saito T, Sawada N, Chiba H. PloS one. 2013 8:e75106.
ATF3 Protects Pulmonary Resident Cells from Acute and Ventilator-Induced Lung Injury by Preventing Nrf2 Degradation. Shan Y, Akram A, Amatullah H, Zhou DY, Gali PL, Maron-Gutierrez T, Gonz[Character e1]lez-L[Character f3]pez A, Zhou L, Rocco PR, Hwang D, Albaiceta GM, Haitsma JJ, Dos Santos CC. Antioxidants & redox signaling. 2014 Mar;22:651-68.
TLR3 deficiency exacerbates the loss of epithelial barrier function during genital tract Chlamydia muridarum infection. Kumar R, Gong H, Liu L, Ramos-Solis N, Seye CI, Derbigny WA. PloS one. 2019 January;14:e0207422.
Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland. Atsuko Y Higashi , Tomohito Higashi, Kyoko Furuse, Kana Ozeki, Mikio Furuse , Hideki Chiba. Scientific reports. 2021 Nov;11:21642.
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Featured Products
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat, Dog
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Dog
Applications:
IHC, IHC - Frozen, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat, Dog
Applications:
Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
