Target
Human OCLN / Occludin
Synonyms
OCLN | Occludin | BLCPMG
Reactivity
Human, Mouse, Rat, Dog
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Purification
Protein A purified
Immunogen
GST fusion protein consisting of the C-Terminus (~150aa) of human occludin.
Specificity
Recognizes human Occludin Protein. Species cross-reactivity: mouse, rat and canine.
Applications
- Immunofluorescence (2 - 10 µg/ml)
- Western blot (0.1 - 1 µg/ml)
- ELISA (0.1 - 1 µg/ml)
Usage
Suitable for use in ELISA, Western Blot and Immunofluorescence. ELISA: 0.1-1 ug/ml. Western Blot: Detects a band at ~65kD. Immunofluorescence: 2-10 ug/ml. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma) and rat liver. Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Short term: 4°C; Long term: -20°C; Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee