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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5737-50 | 50 µl (0.5 mg/ml) | $460 |
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PathPlus™ Polyclonal Rabbit anti‑Human OCLN / Occludin Antibody (aa35‑84, IHC, WB) LS‑B5737
PathPlus™ Polyclonal Rabbit anti‑Human OCLN / Occludin Antibody (aa35‑84, IHC, WB) LS‑B5737
Note: This antibody replaces LS-C110724
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
OCLN (Occludin) is an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Host
Rabbit
Reactivity
Human, Rat
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide located between aa35-84 of human OCLN (Q16625, NP_002529). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%); Opossum, Platypus, Xenopus (92%); Bat (85%); Trout, Zebrafish (81%).
Epitope
aa35-84
Specificity
Human OCLN / Occludin
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About OCLN / Occludin
Validation
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
PathPlus™ OCLN / Occludin Antibody (aa35-84) for IHC, WB/Western LS-B5737 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (1)
TNF ?ARE Pigs: A Translational Crohn's Disease Model. Thomas Winogrodzki, Amira Metwaly, Alessandro Grodziecki, Wei Liang, Bernhard Klinger, Tatiana Flisikowska, Konrad Fischer, Krzysztof Flisikowski, Katja Steiger, Dirk Haller, Angelika Schnieke. Journal of Crohn's & colitis. 2023 July;17:1128-1138.
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Dog
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat, Dog
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat, Dog
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse, Rat, Dog
Applications:
IHC, IHC - Frozen, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat, Dog
Applications:
Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
