Human OCLN / Occludin

Mouse

Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

OC-3F10

Unconjugated. Also available conjugated with APC, Biotin, PE.

Protein A purified

Unmodified

GST fusion protein consisting of the C-Terminus (~150aa) of human occludin.

C-Terminus

Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.

• IHC
• IHC - Paraffin (5 - 10 µg/ml)
• Immunofluorescence (2 - 3 µg/ml)
• Western blot (0.1 - 1 µg/ml)
• ELISA (0.1 - 1 µg/ml)

Immunohistochemistry: LS-B2320 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2320 was determined to be 10 ug/ml. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma). Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q16625 NCBI: NM_002538 NP_002529.1