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OCLN / Occludin

occludin

OCLN / Occludin is an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.

Gene Name: occludin
Synonyms: OCLN, Occludin, BLCPMG
Target Sequences: NM_002538 NP_002529.1 Q16625

Publications (8)

1
The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination With Cell Polarization During Ameloblast Differentiation. Otsu K, Ida-Yonemochi H, Fujiwara N, Harada H. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2016 November;31:1943-1954. (Human) [Full Text Article] [PubMed:27218883] Related Antibodies: LS-B2320.
2
TLR3 deficiency exacerbates the loss of epithelial barrier function during genital tract Chlamydia muridarum infection. Kumar R, Gong H, Liu L, Ramos-Solis N, Seye CI, Derbigny WA. PloS one. 2019 January;14:e0207422. [Full Text Article] [PubMed:30625140] [PMC:PMC6326510] Related Antibodies: LS-B2187.
3
Intestinal epithelial tight junction barrier regulation by autophagy-related protein ATG6/beclin 1. Morgan Wong, Ashwinkumar Subramenium Ganapathy, Eric Suchanec, Laura Laidler, Thomas Ma, Prashant Nighot. American journal of physiology. Cell physiology. 2019 May;316:C753-C765. [Full Text Article] [PubMed:30892937] [PMC:PMC6580157] Related Antibodies: LS-C140253.
4
Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland. Atsuko Y Higashi , Tomohito Higashi, Kyoko Furuse, Kana Ozeki, Mikio Furuse , Hideki Chiba. Scientific reports. 2021 Nov;11:21642. [Full Text Article] [PubMed:34737342] [PMC:PMC8568902] Related Antibodies: LS-B2187.
5
TNF ?ARE Pigs: A Translational Crohn's Disease Model. Thomas Winogrodzki, Amira Metwaly, Alessandro Grodziecki, Wei Liang, Bernhard Klinger, Tatiana Flisikowska, Konrad Fischer, Krzysztof Flisikowski, Katja Steiger, Dirk Haller, Angelika Schnieke. Journal of Crohn's & colitis. 2023 July;17:1128-1138. [Full Text Article] [PubMed:36821422] [PMC:PMC10103242] Related Antibodies: LS-B5737.
6
The tight-junction protein claudin-6 induces epithelial differentiation from mouse F9 and embryonic stem cells. Sugimoto K, Ichikawa-Tomikawa N, Satohisa S, Akashi Y, Kanai R, Saito T, Sawada N, Chiba H. PloS one. 2013 8:e75106. [Full Text Article] [PubMed:24116027] [PMC:PMC3792957] Related Antibodies: LS-B2187.
7
ATF3 Protects Pulmonary Resident Cells from Acute and Ventilator-Induced Lung Injury by Preventing Nrf2 Degradation. Shan Y, Akram A, Amatullah H, Zhou DY, Gali PL, Maron-Gutierrez T, Gonz[Character e1]lez-L[Character f3]pez A, Zhou L, Rocco PR, Hwang D, Albaiceta GM, Haitsma JJ, Dos Santos CC. Antioxidants & redox signaling. 2014 Mar;22:651-68. [Full Text Article] [PubMed:25401197] [PMC:PMC4346377] Related Antibodies: LS-B2187.
8
Protective role of uncoupling protein-2 against dextran sodium sulfate-induced colitis. Zhang H, Kuai XY, Yu P, Lin L, Shi R. Journal of gastroenterology and hepatology. 2012 Mar;27:603-8. [Full Text Article] [PubMed:21871020] Related Antibodies: LS-C50519.
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Proteins (8)
Over-Expression Lysate (2)
Recombinant (6)
OCLN / Occludin (8)
No (8)
GST (1)
GST, N-terminus (1)
His (2)
His, N-Terminal (1)
Myc-DDK (Flag) (3)
Human (6)
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293T Cells (1)
E. coli (3)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (2)
Low endotoxin level (1)
OCLN / Occludin Protein - Purified recombinant protein OCLN was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
59 kDa
20 µg/$1,107
OCLN / Occludin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
59 kDa
20 µg/$150
OCLN / Occludin Protein - Recombinant Occludin By SDS-PAGE
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E. coli
His
11.8 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
OCLN / Occludin Protein - 12.5% SDS-PAGE of human OCLN stained with Coomassie Blue
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Wheat Germ Extract
GST
82.94 kDa
10 µg/$479; 25 µg/$670
OCLN / Occludin Protein - 12.5% SDS-PAGE Stained with Coomassie Blue
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
OCLN / Occludin Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
59 kDa
100 µg/$494
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E. coli
253 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
27.94 kDa
10 mg/$4,268; 5 mg/$3,201; 1 mg/$1,868; 0.5 mg/$1,475; 200 µg/$1,103; 100 µg/$756; 50 µg/$531
OCLN / Occludin Protein
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E. coli
His, N-Terminal
37 kDa
10 µg/$265; 100 µg/$365; 250 µg/$518; 50 µg/$315
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).