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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3442-50 | 50 µl (0.2 mg/ml) | $565 |
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PathPlus™ Monoclonal Mouse anti‑Human TUBB / Beta Tubulin Antibody (IHC, IF, WB) LS‑B3442
PathPlus™ Monoclonal Mouse anti‑Human TUBB / Beta Tubulin Antibody (IHC, IF, WB) LS‑B3442
Note: This antibody replaces LS-C39979
Antibody:
TUBB / Beta Tubulin Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Rabbit, Chicken, Frog
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
TUBB / Beta Tubulin Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Rabbit, Chicken, Frog
Format:
Unconjugated, Unmodified
Specifications
Description
TUBB (beta tubulin class I) is protein that forms a dimer with alpha tubulin to act as a major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain (TUBB) and one at a non-exchangeable site on the alpha chain. Inherited TUBB mutations lead to development brain defects including cortical dysplasia, while somatic mutations are correlated with drug resistance in cancer. In immunohistochemistry, TUBB has variable degrees of cytoplasmic positivity in all tissues.
References: Hum Genet. 1994 Jan;93(1):42-6, PMID: 8270253; Biochem Biophys Res Commun. 2016 Aug 5;476(4):273-279, PMID: 27233604; J Biomol Struct Dyn. 2015;33(12):2530-40, PMID: 26081685; Mol Cancer Ther. 2011 Aug;10(8):1419-29, PMID: 21653684
Target
Human TUBB / Beta Tubulin
Synonyms
TUBB | Beta Tubulin | Beta 5-tubulin | Beta-4 tubulin | Beta1-tubulin | M40 | Tubulin beta-5 chain | Beta Ib tubulin | Class I beta-tubulin | OK/SW-cl.56 | TUBB5 | Tubulin beta chain | Tubulin beta polypeptide | Tubulin beta-1 chain | Tubulin, beta class I | Tubulin, beta polypeptide
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Rabbit, Chicken, Frog
(tested or 100% immunogen sequence identity)
Clonality
IgG3
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Purified tubulin, 55kD. Cellular Localization: Cytoplasmic
Specificity
Recognizes human Tubulin, beta. Species cross-reactivity: Bovine, rabbit, mouse, chicken, rat, frog, moth and wheat.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Immunofluorescence
- Western blot (0.5 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3442 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Western Blot: 0.5-1 ug/ml for 2 hours at RT.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TUBB / Beta Tubulin
Validation
Anti-TUBB / Tubulin, Beta antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-TUBB / Tubulin, Beta antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-TUBB / Tubulin, Beta antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-TUBB / Tubulin, Beta antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
PathPlus™ TUBB / Beta Tubulin Antibody for IHC, IF/Immunofluorescence, WB/Western LS-B3442 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC - Paraffin, ICC, Western blot
Species:
Human, Mouse, Rat
Applications:
Western blot, ELISA
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
