Target
Human TUBB / Beta Tubulin
Synonyms
TUBB | Beta Tubulin | Beta 5-tubulin | Beta-4 tubulin | Beta1-tubulin | M40 | Tubulin beta-5 chain | Beta Ib tubulin | Class I beta-tubulin | OK/SW-cl.56 | TUBB5 | Tubulin beta chain | Tubulin beta polypeptide | Tubulin beta-1 chain | Tubulin, beta class I | Tubulin, beta polypeptide
Reactivity
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Alexa Fluor 555
Immunogen
Synthetic phosphopeptide (KLH-coupled) corresponding to the N-Terminus of human beta-Tubulin.
Specificity
Detects endogenous levels of total human beta-Tubulin protein.
Applications
- IHC
- IHC - Paraffin (1:100)
- ICC
- Immunofluorescence
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ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2189 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2189 was determined to be 1:100. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 2 mg/ml BSA, 0.1% Sodium Azide
Storage
Store at 4°C undiluted. Do not freeze. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TUBB / Beta Tubulin