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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16785-50 | 50 µl (0.5 mg/ml) | $460 |
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PathPlus™ Polyclonal Rabbit anti‑Human NPM1 / NPM / Nucleophosmin Antibody (aa204‑290, IHC, WB) LS‑B16785
PathPlus™ Polyclonal Rabbit anti‑Human NPM1 / NPM / Nucleophosmin Antibody (aa204‑290, IHC, WB) LS‑B16785
Note: This antibody replaces LS-C295691
Antibody:
NPM1 / NPM / Nucleophosmin Rabbit anti-Human Polyclonal (aa204-290) Antibody
Application:
IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
NPM1 / NPM / Nucleophosmin Rabbit anti-Human Polyclonal (aa204-290) Antibody
Application:
IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
Nucleophosmin (NPM1) is a nuclear phosphoprotein that shuttles between the nucleolus and cytoplasm, and is involved in proliferation and growth-suppression pathways. It is involved in ribosome biogenesis, regulates centrosome duplication, and is involved in regulation of the ARF/p53 pathway. Overexpression correlates with cell transformation, and disruption can cause genomic instability and centrosome amplification. When mutated, NPM1 is trapped in the cytoplasm, and the aberrant cytoplasmic location has been used to identify NPM1 mutation status in AML patients. Translocations and fusions with several genes (ALK, RARalpha, MLF1) have been identified in various lymphomas and leukemias.
References: Nat Reviews Cancer 2006 6:493; Ind J Med Res 2016 143(6):763; Heamatologica 2013 98(10):1532; Haematologica 2007 92(4):519
Target
Human NPM1 / NPM / Nucleophosmin
Synonyms
NPM1 | NPM | Nucleolar phosphoprotein B23 | Nucleolar protein NO38 | Numatrin | B23 | Nucleophosmin
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Recombinant NPM (Ala204-Trp290) expressed in E. coli.
Epitope
aa204-290
Specificity
The antibody is a rabbit polyclonal antibody raised against NPM.
Applications
- IHC - Paraffin (10 µg/ml)
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
Presentation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NPM1 / NPM / Nucleophosmin
Validation
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ NPM1 / NPM / Nucleophosmin Antibody (aa204-290) for IHC, WB/Western LS-B16785 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse
Applications:
IHC - Paraffin, ICC, Western blot
Species:
Human
Applications:
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
![NPM1 / NPM / Nucleophosmin Antibody - Immunofluorescence of monoclonal antibody to NPM1 on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/npm1-npm-nucleophosmin-antibody-clone-3b2-ls-c133351/51336_1027566.jpg)
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
