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| Catalog Number | Size | Price |
|---|---|---|
| LS-C37826-250 | 250 µl | $914 |
Monoclonal Mouse anti‑Xenopus NPM1 / NPM / Nucleophosmin Antibody (N‑Terminus, IHC, IF, WB) LS‑C37826
Monoclonal Mouse anti‑Xenopus NPM1 / NPM / Nucleophosmin Antibody (N‑Terminus, IHC, IF, WB) LS‑C37826
Antibody:
NPM1 / NPM / Nucleophosmin Mouse anti-Xenopus Monoclonal (N-Terminus) Antibody
Application:
IHC, IHC-Fr, IF, WB, IP
Reactivity:
Xenopus, Chicken
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
NPM1 / NPM / Nucleophosmin Mouse anti-Xenopus Monoclonal (N-Terminus) Antibody
Application:
IHC, IHC-Fr, IF, WB, IP
Reactivity:
Xenopus, Chicken
Format:
Unconjugated, Unmodified
Specifications
Description
Nucleophosmin antibody LS-C37826 is an unconjugated mouse monoclonal antibody to Nucleophosmin (NPM1 / NPM) (N-Terminus) from xenopus. It is reactive with chicken and xenopus. Validated for IF, IHC, IP and WB.
Target
Xenopus NPM1 / NPM / Nucleophosmin
Synonyms
NPM1 | NPM | Nucleolar phosphoprotein B23 | Nucleolar protein NO38 | Numatrin | B23 | Nucleophosmin
Host
Mouse
Reactivity
Xenopus, Chicken
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Nucleolar fraction prepared from Xenopus laevis oocytes.
Epitope
N-Terminus
Specificity
Recognizes a 38kD nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species). Stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes. Species cross-reactivity: Xenopus laevis, Xenopus borealis, Pleurodelis waltl, Triturus cristatus; XLKE-A6 cells; chicken.
Applications
- IHC
- IHC - Frozen
- Immunofluorescence
- Western blot (1:1000 - 1:2000)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western Blot (Western), Immunoprecipitation (from Xenopus oocyte extracts nucleoplasmin-a 30kD protein-is co-precipitated, which shows high sequence homology to NO38), Immunofluorescence microscopy and Immunohistochemistry (frozen sections, also after fixation with paraformaldehyde). Western Blot: 1:1000-1:2000 (ECL method). Immunohistochemistry: 1:300-1:500; Incubation time 1 hr at RT.
Presentation
0.09% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NPM1 / NPM / Nucleophosmin
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![NPM1 / NPM / Nucleophosmin Antibody - Immunofluorescence of monoclonal antibody to NPM1 on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/npm1-npm-nucleophosmin-antibody-clone-3b2-ls-c133351/51336_1027566.jpg)
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications:
IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Bovine, Rabbit
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Reactivity:
Human
Range:
0.313-20 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
