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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16040-50 | 50 µg (0.5 mg/ml) | $460 |
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IHC‑plus™ Polyclonal Goat anti‑Human NPM1 / NPM / Nucleophosmin Antibody (aa281‑294, IHC, WB) LS‑B16040
IHC‑plus™ Polyclonal Goat anti‑Human NPM1 / NPM / Nucleophosmin Antibody (aa281‑294, IHC, WB) LS‑B16040
Note: This antibody replaces LS-C34661, LS-C73826
Antibody:
NPM1 / NPM / Nucleophosmin Goat anti-Human Polyclonal (aa281-294) Antibody
Application:
IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
NPM1 / NPM / Nucleophosmin Goat anti-Human Polyclonal (aa281-294) Antibody
Application:
IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
Nucleophosmin antibody LS-B16040 is an unconjugated goat polyclonal antibody to Nucleophosmin (NPM1 / NPM) (aa281-294) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NPM1 / NPM / Nucleophosmin
Synonyms
NPM1 | NPM | Nucleolar phosphoprotein B23 | Nucleolar protein NO38 | Numatrin | B23 | Nucleophosmin
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-QEAIQDLWQWRKSL, from the C-Terminus of protein sequence according to NP_002511.1NP_954654.1.
Epitope
aa281-294
Specificity
Human NPM1. This antibody is expected to recognise both report isoforms (NP_002511.1; NP_954654.1).
Applications
- IHC - Paraffin (5 µg/ml)
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx 37 kDa band observed in Human HeLa lysates (calculated MW of 32.6 kDa according to NP_002511.1 and 29.5 kDa according to NP_954654.1). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NPM1 / NPM / Nucleophosmin
Validation
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
IHC-plus™ NPM1 / NPM / Nucleophosmin Antibody (aa281-294) for IHC, WB/Western LS-B16040 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
![NPM1 / NPM / Nucleophosmin Antibody - Immunofluorescence of monoclonal antibody to NPM1 on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/npm1-npm-nucleophosmin-antibody-clone-3b2-ls-c133351/51336_1027566.jpg)
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Xenopus, Chicken
Applications:
IHC, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat, Bovine, Rabbit
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Reactivity:
Human
Range:
0.313-20 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
