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Catalog Number	Size	Price
LS-C661896-10	10 µg	$318
LS-C661896-100	100 µg	$470

TNF Alpha Antibody - Western blot analysis of TNF alpha using anti-TNF alpha antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 25KD. The expected band size for TNF alpha is at 25KD.

Polyclonal Rabbit anti‑Mouse TNF Alpha Antibody (WB) LS‑C661896

Name: Anti-TNF Alpha Antibody | Rabbit anti-Mouse Polyclonal WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C661896-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

TNF Alpha Rabbit anti-Mouse Polyclonal Antibody

Application:

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

$318

LS-C661896-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

TNF Alpha Rabbit anti-Mouse Polyclonal Antibody

Application:

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

TNF Alpha antibody LS-C661896 is an unconjugated rabbit polyclonal antibody to mouse TNF Alpha. Validated for WB.

Target

Mouse TNF Alpha

Synonyms

See All TNF Alpha Antibodies

Host

Rabbit

Reactivity

Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the C-Terminus of mouse TNF alpha (202-235 aa FQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL), different from the related human sequence by five amino acids, and from the related rat sequence by three amino acids.

Applications

Western blot

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About TNF Alpha

Uniprot: P01375 NCBI: NM_000594 NP_000585.2