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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2123-50 | 50 µg (0.1 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Human TNF Alpha Antibody (clone 52B83, IHC, WB) LS‑B2123
IHC‑plus™ Monoclonal Mouse anti‑Human TNF Alpha Antibody (clone 52B83, IHC, WB) LS‑B2123
Note: This antibody replaces LS-C18251
Antibody:
TNF Alpha Mouse anti-Human Monoclonal (52B83) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo, ELISA
Reactivity:
Human, Monkey, Mouse, Guinea pig
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
TNF Alpha Mouse anti-Human Monoclonal (52B83) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo, ELISA
Reactivity:
Human, Monkey, Mouse, Guinea pig
Format:
Unconjugated, Unmodified
Specifications
Description
TNF Alpha antibody LS-B2123 is an unconjugated mouse monoclonal antibody to TNF Alpha from human. It is reactive with human, mouse, guinea pig and other species. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Host
Mouse
Reactivity
Human, Monkey, Mouse, Guinea pig
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
52B83
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Human TNFa.
Specificity
Recognizes human native and recombinant TNF-alpha. Reacts with soluble (17kD) and membrane (26kD) human TNF-alpha. Does not cross-react with TNF-beta, lymphotoxin and receptor bound TNF-alpha.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- IHC - Frozen (1:50)
- Western blot (1:50)
- Flow Cytometry (1:50)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2123 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2123 was determined to be 2.5 ug/ml. Non-specific background staining is observed in connective tissues.
Presentation
PBS, 0.02% Sodium Azide, 0.1% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNF Alpha
Validation
Anti-TNF Alpha antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TNF Alpha antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ TNF Alpha Antibody (clone 52B83) for IHC, WB/Western, Flow, ELISA LS-B2123 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Mouse, Hamster
Applications:
Flow Cytometry, Functional Assay
Species:
Mouse
Applications:
IHC, Western blot, Immunoprecipitation, ELISA, Neutralization, Radioimmunoassay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Date Requested: 12/22/2024
