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| Catalog Number | Size | Price |
|---|---|---|
| LS-C104377-50 | 50 µg | $356 |
| LS-C104377-100 | 100 µg | $396 |
Polyclonal Rabbit anti‑Human TNF Alpha Antibody (WB) LS‑C104377
Polyclonal Rabbit anti‑Human TNF Alpha Antibody (WB) LS‑C104377
Note: This antibody replaces LS-C39990, LS-C54298, LS-C18313, LS-C187685
Antibody:
TNF Alpha Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
TNF Alpha Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
TNF Alpha antibody LS-C104377 is an unconjugated rabbit polyclonal antibody to human TNF Alpha. Validated for ELISA and WB.
Target
Human TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated.
Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human TNF-alpha.
Specificity
Human TNF-alpha
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.5 - 2 µg/ml)
Failed Applications
- Sandwich ELISA
Usage
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.5 ug/ml-1 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human TNF-alpha (0.5 ng/ml), a concentration of 0.08-0.10 ug/ml of this antibody is required. ELISA: To detect Human TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TNF-alpha (LS-C104719) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. Western Blot: To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNF Alpha
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Mouse, Hamster
Applications:
Flow Cytometry, Functional Assay
Species:
Human, Monkey, Mouse, Guinea pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Species:
Mouse
Applications:
IHC, Western blot, Immunoprecipitation, ELISA, Neutralization, Radioimmunoassay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
