Target
Human TLR4
Synonyms
TLR4 | CD284 | CD284 antigen | Homolog of Drosophila toll | TLR-4 | TOLL | ARMD10 | Toll-like receptor 4 | HToll
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
R. Phycoerythrin.
Also available
Unconjugated or conjugated with
Biotin,
FITC.
Purification
Protein G purified
Immunogen
Ba/F3 cell line expressing TLR4 (CD284)
Specificity
Recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. Has been demonstrated to block activation of monocytes with LPS. XXX XXX recommend the use of MCA2061EL for this purpose.
Applications
- Flow Cytometry (1:1)
- Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Method sheets are available on request.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee