Human TLR4

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

HTA125

Unconjugated. Also available conjugated with RPE, Biotin, FITC.

Protein G purified

Unmodified. Also available Low Endotoxin.

Ba/F3 cell line expressing TLR4 (CD284)

Recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. Has been demonstrated to block activation of monocytes with LPS. XXX recommend the use of MCA2061EL for this purpose.

• Western blot
• Immunoprecipitation
• Flow Cytometry (1:10 - 1:25)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Method sheets are available on request.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00206 NCBI: NM_138554 O00206