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| Catalog Number | Size | Price |
|---|---|---|
| LS-C74910-100 | 100 µl | $929 |
Monoclonal Mouse Phosphothreonine Antibody (WB) LS‑C74910
Monoclonal Mouse Phosphothreonine Antibody (WB) LS‑C74910
Antibody:
Phosphothreonine Mouse Monoclonal Antibody
Application:
WB, ELISA
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Phosphothreonine Mouse Monoclonal Antibody
Application:
WB, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
Phosphothreonine antibody LS-C74910 is an unconjugated mouse monoclonal antibody to Phosphothreonine. Validated for ELISA and WB.
Host
Mouse
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Phosphothreonine conjugated to KLH.
Specificity
Reacts specifically with phosphothreonine. Shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphotyrosine. Reacts with free phospho-amino acid, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell Reactivity is specific for phosphothreonine and minimal cross-reactivity is observed against phosphoserine or phosphotyrosine lysates.
Applications
- Western blot (1:500)
- ELISA (1:1000)
Usage
Suitable for use in ELISA and Western Blot. ELISA: 1:1000 to 1:5000, assayed against phosphothreonine conjugated BSA using IgG, H&L (HRP) Pab Gt xMo for detection. Western Blot: 1:500.
Presentation
0.01% sodium azide, 40% glycerol. No buffer or stabilizers added. No stabilizing proteins added.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
![Phosphothreonine Antibody - Anti-Phosphothreonine Antibody - Western Blot. Mab anti-phosphothreonine antibody (clone 18F6) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.](https://lsbio-7d62.kxcdn.com//image2/phosphothreonine-antibody-clone-18f6-ls-c60053/62757_294956.jpg){kind=small}
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/13/2024
Date Requested: 11/13/2024
