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| Catalog Number | Size | Price |
|---|---|---|
| LS-C60053-100 | 100 µl | $574 |
![Phosphothreonine Antibody - Anti-Phosphothreonine Antibody - Western Blot. Mab anti-phosphothreonine antibody (clone 18F6) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.](https://lsbio-7d62.kxcdn.com/image2/phosphothreonine-antibody-clone-18f6-ls-c60053/62757_49223.jpg)
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Monoclonal Mouse Phosphothreonine Antibody (clone 18F6, WB) LS‑C60053
Monoclonal Mouse Phosphothreonine Antibody (clone 18F6, WB) LS‑C60053
Antibody:
Phosphothreonine Mouse Monoclonal (18F6) Antibody
Application:
WB, ELISA
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Phosphothreonine Mouse Monoclonal (18F6) Antibody
Application:
WB, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
Phosphothreonine antibody LS-C60053 is an unconjugated mouse monoclonal antibody to Phosphothreonine. Validated for ELISA and WB.
Host
Mouse
Clonality
IgG1,k
Monoclonal
Clone
18F6
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
Phosphothreonine antibody was produced after repeated immunizations of balb/c mice with phosphothreonine conjugated KLH.
Specificity
This product is clarified ascites produced in balb/c mice using clone 18F6. Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:4000 - 1:20000)
Usage
This monoclonal antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA, and competitive ELISA, with phosphoserine or phosphotyrosine. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, as well as phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases and is important for cell signaling in activation, proliferation and differentiation.
Presentation
Ascites, 0.01% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/28/2025
Date Requested: 1/28/2025
