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| Catalog Number | Size | Price |
|---|---|---|
| LS-C74913-100 | 100 µg | $845 |
Monoclonal Mouse Phosphothreonine Antibody (WB) LS‑C74913
Monoclonal Mouse Phosphothreonine Antibody (WB) LS‑C74913
Antibody:
Phosphothreonine Mouse Monoclonal Antibody
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Phosphothreonine Mouse Monoclonal Antibody
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
Phosphothreonine antibody LS-C74913 is an unconjugated mouse monoclonal antibody to Phosphothreonine. Validated for ELISA, IP and WB.
Host
Mouse
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
Phosphothreonine containing peptides.
Specificity
Species cross-reactivity: Human, Mouse, Rat, Dog. Recognizes a broad range of threonine-phosphorylated proteins.
Applications
- Western blot (1 µg/ml)
- Immunoprecipitation
- ELISA (0.05 µg/ml)
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. ELISA: 0.05 ug/ml. Western Blot: 1 ug/ml for HRPO/ECL detection. Recommended blocking buffer: 3% BSA, 1% PEG 4000, 1% PVP, 0.1% Tween 20, 2x PBS. Immunoprecipitation: 1-10 ug per 1E6 pervanadate-treated A431 cells.
Presentation
Lyophilized from PBS, 0.09% Sodium Azide, Sucrose, PEG
Reconstitution
Reconstitute in 1ml sterile distilled water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
![Phosphothreonine Antibody - Anti-Phosphothreonine Antibody - Western Blot. Mab anti-phosphothreonine antibody (clone 18F6) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.](https://lsbio-7d62.kxcdn.com//image2/phosphothreonine-antibody-clone-18f6-ls-c60053/62757_294956.jpg){kind=small}
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/28/2025
Date Requested: 1/28/2025
