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| Catalog Number | Size | Price |
|---|---|---|
| LS-C132429-100 | 100 µl | $801 |
Monoclonal Mouse Phosphothreonine Antibody (Biotin, WB) LS‑C132429
Monoclonal Mouse Phosphothreonine Antibody (Biotin, WB) LS‑C132429
Antibody:
Phosphothreonine Mouse Monoclonal (Biotin) Antibody
Application:
WB, ELISA
Format:
Biotin, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Phosphothreonine Mouse Monoclonal (Biotin) Antibody
Application:
WB, ELISA
Format:
Biotin, Unmodified
Specifications
Description
Phosphothreonine antibody LS-C132429 is a biotin-conjugated mouse monoclonal antibody to Phosphothreonine. Validated for ELISA and WB.
Host
Mouse
Clonality
IgG
Monoclonal
Conjugations
Biotin
Purification
Ascites
Modifications
Unmodified
Immunogen
o-Phospho-L-threonine (KLH).
Specificity
Recognizes phosphorylated threonine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not react with nonphosphorylated threonine, phosphorylated tyrosine or serine, AMP, or ATP.
Applications
- Western blot
- ELISA (1:5000 - 1:30000)
Usage
Suitable for use in ELISA and Western Blot. ELISA: 1:5000-1:30000. Western Blot: 1:12000-1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide, 0.2% BSA
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Customer Reviews (0)
Featured Products
![Phosphothreonine Antibody - Anti-Phosphothreonine Antibody - Western Blot. Mab anti-phosphothreonine antibody (clone 18F6) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.](https://lsbio-7d62.kxcdn.com//image2/phosphothreonine-antibody-clone-18f6-ls-c60053/62757_294956.jpg){kind=small}
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/28/2025
Date Requested: 1/28/2025
