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| Catalog Number | Size | Price |
|---|---|---|
| LS-C74908-50 | 50 µg | $884 |
Polyclonal Rabbit Phosphothreonine Antibody (WB) LS‑C74908
Polyclonal Rabbit Phosphothreonine Antibody (WB) LS‑C74908
Antibody:
Phosphothreonine Rabbit Polyclonal Antibody
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Phosphothreonine Rabbit Polyclonal Antibody
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
Phosphothreonine antibody LS-C74908 is an unconjugated rabbit polyclonal antibody to Phosphothreonine. Validated for ELISA, IP and WB.
Host
Rabbit
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Phosphothreonine conjugated to KLH and phosvitin.
Specificity
Recognizes phosphothreonine, peptidyl-phosphothreonine and threonine-phosphorylated proteins. Species cross-reactivity: species independent.
Applications
- Western blot (2 µg/ml)
- Immunoprecipitation
- ELISA (0.5 µg/ml)
Usage
Suitable for use in Western Blot, ELISA, and Immunoprecipitation. Western Blot: 2 ug/ml; Mouse brain lysate. Incubate at RT for 4 hrs. ELISA: 0.5 ug/ml (kinase assay). Binding of Ly-pT-Gly-HRP. (0.2 ug/100 ul/well) to an anti-phosphothreonine coated microplate (0.5 ug/100 ul/well) is inhibited by 95% with the addition of 2-10 ug/ml anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/ml.
Presentation
PBS, pH 7, 0.01% sodium azide, 50% glycerol.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
![Phosphothreonine Antibody - Anti-Phosphothreonine Antibody - Western Blot. Mab anti-phosphothreonine antibody (clone 18F6) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.](https://lsbio-7d62.kxcdn.com//image2/phosphothreonine-antibody-clone-18f6-ls-c60053/62757_294956.jpg){kind=small}
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/13/2024
Date Requested: 11/13/2024
