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| Catalog Number | Size | Price |
|---|---|---|
| LS-C528680-100 | 100 µl | $982 |
Monoclonal Mouse Phosphothreonine Antibody (Biotin, WB) LS‑C528680
Monoclonal Mouse Phosphothreonine Antibody (Biotin, WB) LS‑C528680
Antibody:
Phosphothreonine Mouse Monoclonal (Biotin) Antibody
Application:
WB, IP, ELISA
Format:
Biotin, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Phosphothreonine Mouse Monoclonal (Biotin) Antibody
Application:
WB, IP, ELISA
Format:
Biotin, Unmodified
Other formats:
Specifications
Description
Phosphothreonine antibody LS-C528680 is a biotin-conjugated mouse monoclonal antibody to Phosphothreonine. Validated for ELISA, IP and WB.
Host
Mouse
Clonality
IgG2a,k
Monoclonal
Conjugations
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Phosphothreonine containing proteins
Specificity
Recognizes Phosphothreonine, peptidyl-phosphothreonine, and threonine-phosphorylated proteins. Binding of Ly-pT-Gly-HRP (0.2 ug/100 ul/well) to an anti-phosphothreonine coated microplate (0.5 ug/100 ul/well) is inhibited by 95% with the addition of 2-10 ug/ml anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/ml. Species cross-reactivity: species independent.
Applications
- Western blot
- Immunoprecipitation
- ELISA
- Applications tested for the base form of this product only
Presentation
PBS, pH 7.2, No preservatives added
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
![Phosphothreonine Antibody - Anti-Phosphothreonine Antibody - Western Blot. Mab anti-phosphothreonine antibody (clone 18F6) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.](https://lsbio-7d62.kxcdn.com//image2/phosphothreonine-antibody-clone-18f6-ls-c60053/62757_294956.jpg){kind=small}
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/13/2024
Date Requested: 11/13/2024
