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Antibodies > NPM1 / NPM / Nucleophosmin > LS-C800972
 
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Catalog Number Size Price
LS-C800972-100 100 µl (1 mg/ml) $379 
LS-C800972-200 200 µl (1 mg/ml) $421 
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining rat liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining rat liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining rat testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining rat brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining rat brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
NPM1 / NPM / Nucleophosmin Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NPM1 / NPM / Nucleophosmin Antibody - Western blot analysis of Phospho-NPM (Thr199) expression in various lysates
NPM1 / NPM / Nucleophosmin Antibody - Western blot analysis of NPM phosphorylation expression in Jurkat whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining rat liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining rat liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining rat testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining rat brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining rat brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NPM1 / NPM / Nucleophosmin Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
NPM1 / NPM / Nucleophosmin Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NPM1 / NPM / Nucleophosmin Antibody - Western blot analysis of Phospho-NPM (Thr199) expression in various lysates
NPM1 / NPM / Nucleophosmin Antibody - Western blot analysis of NPM phosphorylation expression in Jurkat whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human NPM1 / NPM / Nucleophosmin Antibody (phospho‑Thr199, IHC, IF, WB) LS‑C800972

Polyclonal Rabbit anti‑Human NPM1 / NPM / Nucleophosmin Antibody (phospho‑Thr199, IHC, IF, WB) LS‑C800972

Submit Review LSBio Guarantee
Antibody:
NPM1 / NPM / Nucleophosmin Rabbit anti-Human Polyclonal (pThr199) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800972-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NPM1 / NPM / Nucleophosmin Rabbit anti-Human Polyclonal (pThr199) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Nucleophosmin antibody LS-C800972 is an unconjugated rabbit polyclonal antibody to Nucleophosmin (NPM1 / NPM) (pThr199) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human NPM1 / NPM / Nucleophosmin
Synonyms
NPM1 | NPM | Nucleolar phosphoprotein B23 | Nucleolar protein NO38 | Numatrin | B23 | Nucleophosmin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human NPM around the phosphorylation site of Threonine 199.
Epitope
pThr199
Specificity
Phospho-NPM (Thr199) Antibody detects endogenous levels of NPM only when phosphorylated at Threonine 199.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NPM1 / NPM / Nucleophosmin
P06748 NM_002520 NP_002511.1

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Species: Human
Applications: Immunofluorescence, Western blot, ELISA
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IHC‑plus™ NPM1 / NPM / Nucleophosmin Antibody (aa81‑294, clone 5E3) LS‑B2930
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
NPM1 / NPM / Nucleophosmin Antibody - Detection of mutant nucleophosmin in OCI-AML3 lysates. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NPM1 / NPM / Nucleophosmin Antibody (C‑Terminus, DY650) LS‑C141746
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
NPM1 / NPM / Nucleophosmin Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ NPM1 / NPM / Nucleophosmin Antibody (aa281‑294) LS‑B16040
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Product
NPM1 / NPM / Nucleophosmin Antibody (N‑Terminus) LS‑C37826
Species: Xenopus, Chicken
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
NPM1 / NPM / Nucleophosmin Antibody - Immunohistochemical analysis of Nucleophosmin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NPM1 / NPM / Nucleophosmin Antibody (C‑Terminus) LS‑C413207
Species: Human, Mouse, Rat, Bovine, Rabbit
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
See all NPM1 / NPM / Nucleophosmin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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