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Antibodies > NPM1 / NPM / Nucleophosmin > LS-C782995
 
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Catalog Number Size Price
LS-C782995-100 100 µg (1 mg/ml) $574 
NPM1 / NPM / Nucleophosmin Antibody - IHC staining of FFPE human skin with Nucleophosmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
NPM1 / NPM / Nucleophosmin Antibody - IHC staining of FFPE human colon carcinoma with Nucleophosmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
NPM1 / NPM / Nucleophosmin Antibody - Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Nucleophosmin antibody. These results demonstrate the foremost specificity of the NPM1/1902 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
NPM1 / NPM / Nucleophosmin Antibody - Western blot testing of human 1) MCF7 and 2) A431 cell lysate with Nucleophosmin antibody. Expected molecular weight ~38 kDa.
NPM1 / NPM / Nucleophosmin Antibody - Western blot testing of human A431 cell lysate with Nucleophosmin antibody. Expected molecular weight ~38 kDa.
NPM1 / NPM / Nucleophosmin Antibody - IHC staining of FFPE human skin with Nucleophosmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
NPM1 / NPM / Nucleophosmin Antibody - IHC staining of FFPE human colon carcinoma with Nucleophosmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
NPM1 / NPM / Nucleophosmin Antibody - Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Nucleophosmin antibody. These results demonstrate the foremost specificity of the NPM1/1902 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
NPM1 / NPM / Nucleophosmin Antibody - Western blot testing of human 1) MCF7 and 2) A431 cell lysate with Nucleophosmin antibody. Expected molecular weight ~38 kDa.
NPM1 / NPM / Nucleophosmin Antibody - Western blot testing of human A431 cell lysate with Nucleophosmin antibody. Expected molecular weight ~38 kDa.
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Monoclonal Mouse anti‑Human NPM1 / NPM / Nucleophosmin Antibody (clone NPM1/1902, Azide‑free,BSA‑free, IHC, WB) LS‑C782995

Monoclonal Mouse anti‑Human NPM1 / NPM / Nucleophosmin Antibody (clone NPM1/1902, Azide‑free,BSA‑free, IHC, WB) LS‑C782995

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Antibody:
NPM1 / NPM / Nucleophosmin Mouse anti-Human Monoclonal (Azide-free, BSA-free) (NPM1/1902) Antibody
Application:
IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Unmodified
Price
Catalog Number
$574
LS-C782995-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NPM1 / NPM / Nucleophosmin Mouse anti-Human Monoclonal (Azide-free, BSA-free) (NPM1/1902) Antibody
Application:
IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Unmodified

Specifications

Description
Nucleophosmin antibody LS-C782995 is an unconjugated mouse monoclonal antibody to human Nucleophosmin (NPM1 / NPM). Validated for IHC and WB.
Target
Human NPM1 / NPM / Nucleophosmin
Synonyms
NPM1 | NPM | Nucleolar phosphoprotein B23 | Nucleolar protein NO38 | Numatrin | B23 | Nucleophosmin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
NPM1/1902
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Azide-free, BSA-free. Also available Unmodified.
Immunogen
A portion of amino acids 185-287 from the human protein was used as the immunogen for the Nucleophosmin antibody.
Specificity
This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis.
Applications
  • IHC - Paraffin (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Optimal dilution of the Nucleophosmin antibody should be determined by the researcher. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Presentation
1X PBS
Storage
Aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NPM1 / NPM / Nucleophosmin
P06748 NM_002520 NP_002511.1

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Featured Products

NPM1 / NPM / Nucleophosmin Antibody - Immunofluorescence of monoclonal antibody to NPM1 on HeLa cell. [antibody concentration 10 ug/ml]
NPM1 / NPM / Nucleophosmin Antibody (clone 3B2) LS‑C133351
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
NPM1 / NPM / Nucleophosmin Antibody - Detection of mutant nucleophosmin in OCI-AML3 lysates. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NPM1 / NPM / Nucleophosmin Antibody (C‑Terminus, DY650) LS‑C141746
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
NPM1 / NPM / Nucleophosmin Antibody - Anti-NPM1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ NPM1 / NPM / Nucleophosmin Antibody (aa81‑294, clone 5E3) LS‑B2930
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
NPM1 / NPM / Nucleophosmin Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ NPM1 / NPM / Nucleophosmin Antibody (aa281‑294) LS‑B16040
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Product
NPM1 / NPM / Nucleophosmin Antibody (N‑Terminus) LS‑C37826
Species: Xenopus, Chicken
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
NPM1 / NPM / Nucleophosmin Antibody - Immunohistochemical analysis of Nucleophosmin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NPM1 / NPM / Nucleophosmin Antibody (C‑Terminus) LS‑C413207
Species: Human, Mouse, Rat, Bovine, Rabbit
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
See all NPM1 / NPM / Nucleophosmin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 10/31/2024
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